金雀花组织培养与快繁

Tissue culture and rapid propagation of Caragana sinica

以金雀花Caragana sinica茎段为材料,通过不同植物生长调节物质种类和质量浓度进行单因素设计,建立金雀花的组织培养再生体系,为金雀花的组织快繁提供技术指导。结果表明:用1.0 g.L-1的氯化汞(HgCl2)浸泡灭菌10 min为宜;初代培养宜选用培养基MS(Murashige and Skoog)+2.0 mg.L-16-苄基腺嘌呤(6-BA)+0.2 mg.L-1萘乙酸(NAA);最佳增殖培养基为MS中添加0.5 mg.L-1苯基噻二唑基脲(TDZ),诱导形成5～6个丛生芽,将嫩茎接种在添加0.5 mg.L-1NAA的MS培养基中,生根率为86.7%。

Caragana sinica is a edible flower,having good market demand.This aim is to establish a efficient tissue culture system for its commercial propagation.The stem was taken as the explants,this experiment adopted the single factor experimental design,the effects of different plant growth regulator treatments on various processes of tissue culture were studied.Results were as follows：the optimal sterilization method for the explant was dipping in 1.0 g·L-1 HgCl2 for about 10 min.The contamination rate（36.7%） and browning rate（16.7%） were better than other treatments.The Murashige and Skoog（MS） culture media containing 2.0 mg·L-1 6-benzyladenine（BA） ＋ 0.2 mg·L-1 α-naphthalene acetic acid（NAA） was suitable for the initial culture,the induction rate was 60.0%（P＆lt;0.05）.For proliferation,the optimal medium was MS supplemented with 0.5 mg·L-1 thidiazuron（TDZ） with 5-6 clustered buds being induced,the multiplication rate could reach 4.5（P＆lt;0.05）.When shoots were inoculated with MS supplemented with 0.5 mg·L-1 NAA,the rooting rate was up to 86.7%.The results could provide technical guidance for large-scale micropropagation of Caragana sinica.