某海训官兵幽门螺杆菌感染及其基因型调查分析

Helicobacter pylori infection among naval personnel of a certain unit undergoing training at sea as well as investigation and analysis of its genotype

目的了解某沿海地区参加海训官兵幽门螺杆菌(Hp)感染及其基因型情况。方法随机抽查参加海上训练的官兵516人,问卷调查年龄、职别、部别、疾病史,按有无晕船症状分为2组,晕船组146人,无晕船组370人;采用免疫层析金标记技术对所有受检者进行血清Hp抗体检测;对Hp抗体阳性者进一步采用免疫印迹法检测血淸Hp CagA、VacA、UreB、UreA抗体。结果 Hp抗体总体阳性率36.6%(189/516),其中晕船组Hp抗体阳性率65.1%(95/146),无晕船组Hp抗体阳性率25.4%(94/370),两者比较差异有统计学意义(P<0.01)。Hp抗体阳性无晕船组Hp基因抗体表达分别为CagA 29.8%(28/94)、VacA 39.4%(37/94)、UreA 39.4%(37/94)、UreB 43.6%(41/94);晕船组Hp基因抗体表达分别为CagA 63.2%(60/95)、VacA 55.8%(53/95)、UreA 31.6%(30/95)、UreB 26.3%(25/95);晕船组Hp CagA及VacA抗体阳性表达分别明显高于UreA抗体阳性表达(P<0.01),并明显高于无晕船组;且晕船组Hp VacA抗体表达高于UreB抗体表达(P<0.01)。随着年龄增长,CagA、VacA阳性率明显增加(P<0.05或P<0.01)。结论海训官兵Hp总体感染率与当地驻军官兵无明显差异,但晕船组Hp感染率及Hp CagA、VacA基因抗体阳性率均明显高于无晕船组,且与年龄相关。

Objective To investigate Helicobacter pylori（Hp） infection among naval personnel of a certain unit on the coastal region of Quanzhou, who underwent training at sea and also to study its genotype. Methods Five hundred and sixteen naval personnel who underwent training at sea were randomly selected for the study. Questionnaire was conducted on the age, rank, unit designation, medical history and motion sickness symptoms among the naval personnel, who were then randomly divided into two groups： the motion sickness group （n = 146） and the non-motion sickness group （ n = 370）. Serum Hp antibody detection was made with immune chroma- tography gold marker. Then, Hp gene （CagA, VacA, UreB, UreA） antibodies were further detected by Western blot for those Hp anti- body positive cases. Results Total Hp antibody positive rate was 36.6% （189/516）. Of all the cases, Hp antibody positive rate of the motion sickness group was 65.1% （95/146） and Hp antibody positive rate of the non-motion sickness group was 25.4% （94/370）. As shown above, statistical significance could be noted, when comparisons were made between them （P 〈 0. 01 ）. Hp gene antibody posi- tive expression levels for the non-motion sickness group were CagA 29.8% （28/94）, VacA 39.4% （37/94）, UreA 39.4% （37/94） and UreB 43.6% （41/94）respectively, while on the other hand, Hp gene antibody positive expression levels for the motion sickness group were CagA 63.2% （60/95）, VacA 55.8% （53/95）, Urea 31.6% （30/95）and UreB 26.3% （25/95）. Hp gene antibody positive expression levels of CagA 29.8% （28/94） and VacA 39.4% （37/94）were all significantly higher than that of Urea （P 〈0.01 ）, and they were obviously higher than those of the non-motion sickness group. Hp antibody positive expression level of CagA was higher than that of UreB （P 〈 0.01 ）. With the growing of one＇s age, positive expression rates of CagA and VaeA increased significantly （P 〈 0.05 or P 〈 0.01 ）. Conclusion No significant differences could be seen in total Hp infection rate （ P 〈 0.01 ） between naval person- nel who underwent training at sea and the local garrison forces. However, Hp infection rate of the motion sickness group and Hp anti- body positive expression rates of CagA, VacA were all significantly higher than those of the non-motion sickness group.