摘要
目的探讨氧化低密度脂蛋白(ox-LDL)对树突状细胞表面CD36表达的影响。方法梯度离心法分离人外周血单核细胞,经含重组人粒细胞-巨噬细胞集落刺激因子和重组人白介素4的DC完全培养液中培养,使其分化为DC。DC分别与不同浓度ox-LDL(分别为10μg/ml、50μg/ml)共同孵育48 h后,镜下观察DC形态变化,同时采用流式细胞术检测DCs表型(CD1α、CD83)和CD36的表达,以及用ELISA法检测细胞上清液中IL-10、IL-12含量。结果与对照组相比,ox-LDL 50μg/ml组DCs表型CD1α、CD83以及CD36的表达明显上调(p<0.01),ox-LDL 50μg/ml组细胞上清液中IL-10、IL-12含量明显分别明显升高和降低(p<0.01)。结论 DCs表面CD36的表达与ox-LDL诱导的DCs成熟以及炎症因子的释放密切相关,CD36可能在DCs分化成熟以及抗原递呈过程中发挥重要作用。
Objective To investigate the effect of ox - LDL on CD36 expression on the surface of dendritic cell. Methods Human monoc-ytes were isolated by using gradient centrifugation method and cultured in CellGro DC medium containing recombinant hu-man granulocyte-macrophage colony -stimulating factor （ rhGM-CSF） and recombinant human interleukin-4 （rh IL-4）, and then DCs were obtained. DCs were treated with oxidized ox-LDL （10 μg/ml, 50 μg/ml, respectively） for 48 hours. The morphologica/ changes were observed under the phase contrast microscope, the phenotypes of DCs, including CDla and CD83, and CD36 were detec-ted by flow cytometry, and the content of IL-10 and IL - 12 in cell cuture supematant were quantitative detected by ELISA. Results Compared with control group, the expression of DCs phenotype CDletand CD83, and CD36 were upregulated in ox - LDL 50 μg/ml group（p 〈0. 01 ）, the content of IL - 10 and IL - 12 in cell cuture supematant were upreguated and downregulated in ox - LDL 50μg/rrd greup（p 〈0. 01 ）, respectively. Conclusion The expression of CD36 on DCs surface is closely related with the maturation and inflammatory cytokine secretion of DCs induced by ox - LDL. CD36 may play a important role in the differentiation and maturation and antigen - oresent oroeess of DCs.
出处
《现代医院》
2013年第8期28-30,共3页
Modern Hospitals
基金
国家自然科学基金青年科学基金项目(编号:81202951)
