中华绒螯蟹DNA指纹图谱的初步研究

Tentative Studies on DNA Fingerprinting of Chinese Mitten－ handed Crabs(Eriocheir sinensis)

本文运用 DIG标记的人源小卫星探针 33.6与 HaeⅢ消化的中华绒螯蟹基因组 DNA杂交，建立了中华绒螯蟹非放射性 DNA指纹图谱，证明目前在缺乏来自中华绒螯蟹基因组 DNA本身的同源小卫星 DNA的情况下，运用异源小卫星探针进行中华绒螯蟹 DNA指纹图谱的研究是可行的；实验表明运用人源小卫星探针 33.6进行中华绒螯蟹 DNA指纹图谱研究时，该探针可检测到 10个以上位点的遗传变异，每个位点呈现丰富的多态性。

The studides established non－ radioactive DNA fingerpringting of Chinese mitten－ handed crabs(Eriocheir sinensis) by hybridizing HaeⅢ completely dogested genomic DNA of Chinese mitten－ handed crabs with DIG labeled probe of human mini－ satellite 33.6.It showed that studying on DNA fingetpringting of Chinese mitten－ handed crabs with non－ homologous mini－ satellite probes was feasible in short of homologous mini－ satellite probes cloned from gDNA of Chinese mitten－ handed crabs.DNA fingerpringting of Chinese mitten－ handed crabs produced by human mini－ satellite 33.6 detected over 10 locus in gDNA of Chinese mitten－ handed crabs and those locus showed abundence of polymorphism.