玉米谷胱甘肽过氧化物酶生物信息学分析

Bioinformatics Analysis of Glutathione Peroxidase in Zea Mays

谷胱甘肽过氧化物酶(GPX)是生物体内重要的活性氧自由基清除剂,它能够清除生物体内的过氧化氢和脂质过氧化物,阻断活性氧自由基对机体的进一步损伤,保证生物体能正常进行生命活动。以玉米谷胱甘肽过氧化物酶基因家族的11个成员为研究对象,对其编码的蛋白质的结构和功能进行分析,包括等电点、分子量、亲水性值、二级结构和亚细胞定位等,并建立了分子系统进化树。结果发现,玉米谷胱甘肽过氧化物酶基因家族的11个成员的等电点和相对分子量存在差异,而二级结构存在相似特征,其中,二级结构包括α-螺旋、β-折叠、β-转角和无规则卷曲。以上分析为全面解析玉米谷胱甘肽过氧化物酶的功能奠定了基础,并可为植物抵御氧化胁迫研究提供理论依据。

The GPX（Glutathione Peroxidase） is important active oxygen free radical scavengers in biosome,which can remove hydrogen peroxide and lipid peroxides,block active oxygen free radical to damage the body,and ensure the normal biological activities.In this study,the structure and function of GPX family genes encoding protein in Zea Mays such as isoelectric point,molecular weight,the number of amino acids,hydrophilic property,secondary structure and subcellular localization were analyzed,and the phylogenetic tree was built by a series of bioinformatics software.The results showed that： the diversity characteristic of isoelectric point and molecular weight was observed among these GPX genes,and the similar characteristics such as secondary structure was observed.The secondary structure included α-helix,β-sheet,β-turn and random coil.The above results lay a foundation for comprehensive analysis of GPX in Zea Mays and provide theoretical basis for the resisting oxidative stress.