围心脏不停跳体外循环期猪血液流变性变化

Changes of Porcine Hemorrheological Parameters during Cardiopulmonary Bypass on Beating Heart

目的:观察心脏不停跳体外循环猪血液流变学指标变化。方法:选择猪龄3～4月、体重20～25Kg的广西本地健康杂种猪10只,全身麻醉后建立心脏不停跳体外循环模型。于转机开始前(T1)、低温维持30min(T2)、复温完成停机前(T3)和停机后20min(T4),先经股动脉采动脉血5ml为动脉血组(A组),1min后经中心静脉导管采静脉血5ml为静脉血组(V组),测定其低切变率(10s-1)、中切变率(50s-1)及高切变率(150s-1)全血表观粘度(ηb)和全血还原粘度(ηre),以及红细胞聚集指数(RAI)、红细胞刚性指数(IR)、红细胞变形指数(TK)和红细胞压积(Hct),分析两组组内和组间各时点血液流变学指标的变化。结果:两组组内T2～T4各切变率的ηb、ηre及Hct、RAI均低于T1水平(P<0.05),但组间差异无统计学意义(P>0.05);各时点IR、TK水平变化在组内、组间的差异无统计学意义(P>0.05)。结论:广西本地健康杂种猪在心脏不停跳体外循环期间血液流变性变化有利于微循环灌注。

Objective: To investigate the effects of porcine hemorrheological indices during cardiopulmonary bypass (CPB) on beating heart. Method: 10 Guangxi local health cross-bred pigs that the age was 3～4 months and the weight was 20～25Kg were choosed to establish the experiment model of CPB on beating heart after general anesthesia. Blood samples were taken before CPB(T1),low temperature maintain 30min(T2),finish rewarming before the end of CPB(T3)and 20min after the end of CPB(T4),first draw 5ml blood from femoral artery as artery blood group(group A),after 1 min draw 5ml blood from central venous catheter as vein blood group(group V). The specimen’s hemorheology indices included blood viscosity(ηb) and blood viscosity reduction(ηre) in 10s-1,50s-1,150s-1 ,erythrocyte aggregation (RAI),RBC rigid index (IR) and erythrocyte deformation index(TK) and hematocrit (Hct),et al were detected. Results: The levels of two groups’ indices such as ηb,ηre,Hct and RAI were all lower in T2, T3, and T4 time points than in T1 time point(P<0.05), but there was no significant difference between the two groups; The changes of IR and TK both in the group and in between groups had no significant difference(P>0.05). Conclusion: In the model of Guangxi local health cross-bred pigs established on beating heart under cardiopulmonary bypass, changes of hemorrheological parameters during cardiopulmonary bypass present good advantage for microcirculation perfusion.