乙型肝炎“小三阳”患者HBV-DNA定量检测与前S1抗原的关系

Relationship Between HBV-DNA and Pre S1 Antigen in Patients with HBsAg,Anti-HBe and Anti-HBc Positive Hepatitis B

目的探讨"小三阳"患者HBV-DNA含量与前S1抗原的关系。方法收集乙型肝炎"小三阳"患者血清152例,用ELISA方法检测前S1抗原,用实时荧光定量PCR(FQ-PCR)方法检测HBV-DNA。结果前S1与HBV-DNA同时阳性者74例,二者同时阴性者34例,符合率为71.1%。前S1阳性、HBV-DNA阴性者为30例,HBV-DNA阳性而前S1阴性者为14例。即"小三阳"患者中前S1的检出率为68.4%,HBV-DNA的检出率为57.9%,并且拷贝数高低有差别。结论 "小三阳"患者体内也存在着不同程度的病毒复制。前S1的检测不能代替HBV-DNA,只能作为其补充指标。对于"小三阳"患者,同时检测HBV-DNA定量有助于判断病毒在体内是否复制。

Objective To investigate the relationship between HBV-DNA with pre S1 antigen in patients with HBsAg, Anti- HBe and Anti- HBc positive hepatitis B. Methods The serum express of pre S1 antigen and HBV- DNA in 152 patients with HBsAg, Anti-HBe and Anti-HBc positive hepatitis B were detected by ELISA and PCR（FQ-PCR） respectively. Results 74 cases of samples were both pre S1 and HBV-DNA positive, while 34 cases were found Pre S1 and HBV-DNA negative. The positive coincidence rate between Pre S1 and HBV-DNA was 71.1%. There were 30 cases with pre S1 positive and HBV-DNA negative, while 14 cases had HBV-DNA positive and pre S1 negatiye. The detection rate of pre S1 and HBV-DNA in patients with HBsAg, Anti-HBe and Anti-HBc positive hepatitis B were 68.4% and 57.9% , respectively. Conclusion There are virus replica- tions of different degrees in patients with HBsAg, Anti-HBe and Anti-HBc positive hepatitis B. The detection of Pre S1 can not replace HBV-DNA detection, and it could be only used as a supplementary index. The HBV- DNA quantitative detection could facilitate to judge whether virus in-vivo replicate or not.