不同长度疟原虫环子孢子蛋白重复序列DNA免疫的实验分析

A Preliminary Analysis on the DNA Vaccination with Different Sizes of Repeat Sequences of Circumsporozoite Protein Gene

本实验利用DNA免疫技术 ,将PCR得到的疟原虫环子孢子蛋白 (CSP )不同长度的重复序列基因克隆到pcDNA3质粒中 ,对小鼠进行肌肉注射 ,经过免疫检测 ,结果证明不同长度CSP的重复序列能够单独诱发机体全面的细胞免疫和体液免疫 ,这为今后疟疾疫苗的发展提供了新的思路。

The different sizes of repeat sequences encoding the CSP of Plasmodium falciparium were cloned into plasmid pcDNA3 and BALB/c mice were inoculated intramuscularly with this recombinant plasmid DNA It was found that the vaccination with this plasmid DNA could elicite both humoral and cellular immune responses These results privide a new way of approaches to the reseach of human malaria vaccines