新疆地区COPD急性发作期及稳定期血清中性粒细胞弹性蛋白酶、α1-抗胰蛋白酶变化的研究

Study on the Changes of Elastinase and α1-Antitrypsin in Serum Neutrophile Granulocyte of COPD Patients at Acute Exacerbation and Stable Stage in Xingjiang Province

【目的】探讨蛋白酶／抗蛋白酶失衡在慢性阻塞性肺疾病（COPD）发生、发展过程中的机制，以及弹性蛋白酶（NE）和α1-抗胰蛋白酶（α1-AT）与COPD疾病的相关性，炎症因子变化的过程。【方法】对85例cOPD患者急性加重期和稳定期的血清使用酶联免疫吸附法（El。ISA）法测定NE和α1-AT的血清浓度。【结果】急性加重期血清NE浓度明显增高（P〈0．05）；COPD稳定期组和对照组比较以及COPD急性加重期组和COPD稳定期组血清NE浓度比较，差异无统计学意义；血清α1-AT在cOPD急性加重期组水平显著高于稳定期（P〈O．005）与对照组（P〈0．01），而稳定期组又显著高于对照组（P〈0．05）。【结论】蛋白酶／抗蛋白酶失衡在COPD急性加重期及稳定期均存在，是COPD发生发展的重因素。

[Objective]To explore the mechanism of protease/antiprotease unbalance in the pathogenesis of chronic obstructive pulmonary disease（COPD）, the correlation of neutrophil（NE） and α1-antitrypsin（al-AT） with COPD and the process of the changes of inflammatory factors. [Methods]Enzyme-linked immunosorbent assay（ELfSA） was used to detect the level of NE andcd AT in serum of 85 patients with COPE） at acute exac- erbation and stable stage. [Results] Serum NE level at acute exacerbation stage obviously increased（ P 〈 0.05）. There was no significant difference in serum NE level between COPE） stable stage group and control group or COPD acute exacerbation group and COPD stable stage group. Serum level of α1-AT in COPE） acute exacerbation group was significantly higher than that in stable stage group（ P 〈0. 005） and control group（ P 〈0.01）, while that in stable stage group was significantly higher than that in control group（ P 〈0. 05）. [Conclusion] Protease/antiprotease unbalance exists in both acute exacerbation and stable stage of COPE） pa- tients. It is important factor in the pathogenesis and development of COPE）.