大鼠慢性萎缩性胃炎癌前病变气虚血瘀证动物模型的研制

Rat Model of Qi-deficiency-blood-stasis Syndrome of Precancerous Lesion Developed from Chronic Atrophic Gastritis

目的 探讨慢性萎缩性胃炎 (CAG)癌前病变气虚血瘀证动物模型的研制方法。方法 2 5只大鼠随机分为 2组 ,正常对照组不予造模 ,造模组采用甲硝基亚硝基胍 (MNNG)溶液自由饮用 ,雷尼替丁灌胃 ,饥饱失常的综合法造模 2 0周 ,观察 2组大鼠周身状况 ,血液红细胞免疫功能、LPO、SOD、GSH -Px、TXB2 、6 -keto -PGF1α、TXB2 / 6 -keto -PGF1α及胃粘膜病理等变化。结果 模型组大鼠周身状况较差 ,肝、脾、胸腺重量减轻 ,血液RBC -IC花环率、LPO、TXB2 、6 -keto -PGF1α含量及TXB2 / 6 -keto -PGF1α比值增高 ,RBC -C3b受体花环率、SOD、GSH -Px活力降低 ,肉眼、光镜及电镜观察胃粘膜形态均有典型的CAG癌前病变病理改变。结论 该动物模型经多学科、多指标观察 ,与临床CAG癌前病变气虚血瘀证表现有较好的一致性 ,稳定可靠 ,重复性好 。

OBJECTIVE To study the technique for constructing an animal model of qideficiencybloodstasis syndrome of precancerous lesion developed from chronic atrophic gastritis (CAG). METHODS 25 rats were divided randomly into 2 groups. The rats of the control group were fed normally. The rats in the model group were provided with MNNG solution for drinking freely, ranitidine was introduced to their stomach, and they were sometimes starved and sometimes overfed. 20 weeks later, the general health, immunity of red cells, the indices of LPO, SOD, GSHPx, TXB 2, 6ketoPGFla, TXB 2/6ketoPGFla and pathological change of gastric mucosa of both groups were observed. RESULTS The general health of the model group was poor. The weights of their liver, spleen and thymus were lowered. The blood RBCIC rosette forming rate, the contents of LPO, TXB 2, 6ketoPGFla and the ratio of TXB 2/6ketoPGFla were all increased. The RBCC3b receptor rosette forming rate and the activities of SOD and GSHPX were lowered. There were typical precancerous pathological morphological changes in the gastric mucosa, which were observed by naked eyes, through light microscope and through electron microscope. CONCLUSION The multidiscipline and multiindex observation shows the symptoms of the animal model are consistent with clinical manifestations of qideficiencybloodstasis syndrome of precancerous lesion developed from CAG.The model is reliable and repeatable. It has provided a good animal model for the study of traditional Chinese medicine in treating precancerous lesion developed from CAG.