化疗大鼠脾切除前后免疫功能改变的实验研究

The changes of immune function before and after splenectomy in mice with chemotherapy

目的 探讨接受化学药物治疗后脾切除前后免疫功能的变化。方法 本实验将 72只大鼠随机分为 3组 :(1)对照组 ;(2 )激素负荷组 ;(3)免疫抑制组。脾切除前后测定多项免疫指标 :T淋巴细胞增殖能力、NK细胞活性和中性粒细胞吞噬功能。结果 脾脏切除前激素负荷组和免疫抑制组 ,T淋巴细胞增殖能力、NK细胞活性和中性粒细胞吞噬功能皆明显低于对照组 (P <0 .0 1) ;脾切除后对照组上述免疫指标下降 (P <0 .0 1) ,而激素负荷组和免疫抑制组变化不大 (P >0 .0 5 )。结论 术前大量应用激素或免疫抑制剂所引起的免疫功能损害及其对术后的影响 ,是导致机体脾切除术后免疫功能降低的主要原因 ,而切除有免疫功能障碍的脾脏可能是次要因素。

Objective To search for the changes of immune function before and after splenectomy in mice models with chemotherapy. Methods Seventy two mice were divided equally into three groups as follows: (1) control group; (2) steroid loading group; and (3) immune suppressive group. The immune function was examined before and after splenectomy. Results The lymphocyte proliferation, natural killer cell activity and neutrophil phagocytosis in group (2) and (3) were significant lower than that of group (1) ( P < 0.01 ). Above immune function index were decreased in group (1) ( P < 0.01 ), and no difference was observed in group (2) and (3) postoperatively ( P < 0.05 ). Conclusion Using large dosage of glucocorticoids or immune suppressive drugs is the main factor leading to the immunosuppression after splenectomy.