荧光定量检测HBV DNA与乙肝两对半结果对比分析研究

Quantitative detection of HBV DNA by FQ-PCR and comparative analysis of its results with HBV-M by ELISA

目的 为了对乙肝患者体内 HBV复制及传染性有更直接地了解 ,亦更有利于对临床 HBV感染的诊断、治疗方案的选择及疗效判定。方法 运用荧光定量 PCR(FQ- PCR)和 EL ISA两种方法同时检测了 310份肝炎患者血清 ,并对结果进行了对比分析。结果 乙肝大三阳患者血清 HBV DNA检出率为 92 .5 % (74/ 80 ) ,平均拷贝数为 4.10× 10 1 0 /ml;小三阳患者血清 HBV DNA检出率为 32 .35 % (2 2 / 6 8) ,平均拷贝数为 1.31× 10 9/ ml;HBs Ag(+ )、HBc Ab(+ )组血清 HBV DNA检出率为 45 .71% (32 / 70 ) ,平均考贝数为 4.0 8× 10 8ml;HBV- M全阴性组血清 HBV DNA检出率仍达6 .6 7% (2 / 32 ) ,平均拷贝数仍达 1.5 4× 10 8/ ml。结论 HBV- M阴性患者仍有 HBV DNA阳性者 ,因此对临床 HBV感染、复制及传染性的判断以及指导治疗 ,除乙肝二对半标志物 EL ISA检测外 ,加做 HBV DNA FQ- PCR检测同样具有重要意义。

Objective To gain in knowledge about the HBV replication and infectivity in patients with hepatitis B and hence to facilitate the selection of approaches to diagnosis,treatment,and assessment of curative effect.Methods The serum samples from 310 cases of viral hepatitis were tested by Fluorescence quantitative PCR assay(FQ PCR),and also by ELISA as contrast.Results In 74 of 80 cases with HBsAg(+),HBeAg(+),HBcAb(+)sample,FQ PCR results were positive and the load of HBV DNA was 4.16×10 10 copies/ml,with a positive rate of 92.50.In 22 of 68 cases with HBsAg(+),HBeAb(+) and HBcAb(+) samples,the average load was 1.31×10 9 copies/ml,with a positive rate of 32.35%. In 32 of 70 cases of HBsAg(+) and HBcAb(+) samples,the average HBV DNA load was 4.04×10 8copies/ml,with a positive rate of 45.71%.In 2 of 32 cases of HBV M negative,the positive rate of HBV DNA was 6.67%.Conclusion The results showed that HBV DNA could possibly be positive even though it might be negative for HBV M.FQ PCR could be used as another good monitor for the state of HBV infection and its complication.