丹皮酚对脂质过氧化反应及低密度脂蛋白氧化修饰的抑制作用

Inhibitory Effect of Paeonol on Lipid Peroxidational Reaction and Oxidational Decorate of Low Density Lipoprotein

目的 :观察丹皮酚 (以下简称Pae)对脂质过氧化反应及低密度脂蛋白 (LDL)氧化修饰的抑制作用。方法 :采用含胆固醇高脂膳食喂养大鼠 6周形成高脂血症模型 ,硫代巴比妥酸 (TBA)比色法和酶标多克隆抗体夹心法分别测定丙二醛 (MDA)和氧化低密度脂蛋白 (ox LDL) ,改良沉淀法分离健康人血清LDL。结果 :Pae(30 0 ,15 0mg·kg-1)可明显抑制高脂大鼠血清、主动脉及肝脏脂质过氧化反应 ;Pae(30 0mg .kg-1)显著减少高脂大鼠血浆LDL的氧化修饰 ;Pae(10 0 0 ,2 0 0 ,40 μg .ml-1)对健康人血清LDL的体外氧化反应有极显著抑制效应。结论 :Pae降低高脂血症大鼠MDA及ox LDL水平 ,抑制健康人血清LDL的体外氧化修饰反应。

Objective:To observe the effects of paeonol(Pae)on lipid peroxidational reaction and oxidational decorate of low density lipoprotein(LDL).Method:The rat model of hyperlipidaemia was established by feeding high lipid diet with cholesterol for 6 weeks.The contents of malondialdehyde(MDA)and oxidized low density lipoprotein(OX LDL)were measured by thiobarbituric acid reactive substance(TBA)method and sandwich method of enzyme linked immunosorbent assay for detection of polyclone antibody.LDL of serum in healthy person was separated by improve precipitate method.Results:Pae(300,150 mg·kg -1) could reduce significantly the levels of MDA of serum,aorta and liver;Pae(300 mg.kg -1 )decreased obviously the content of OX LDL of plasma;Pae(1 000,200,40 μg·ml -1 )refrained markadly the course of oxidational decorate of LDL of serum in healthy person.Conclusion:Pae could reduce the levels of MDA and OX LDL in hyperlipidaemia rats,and refrain the oxidational decorates of LDL of serum in healthy person. [