氯过氧化物酶对苯酚生物降解的促进作用

Study on the promoted biodegradation of phenol by chloroperoxidase

氯过氧化物酶是一种底物广泛的手性催化剂,可以催化卤素离子、芳香族化合物和醇类化合物等进行过氧化反应。利用氯过氧化物酶催化氧化苯酚,考察其对苯酚生物降解的促进作用。结果表明,500、1 000mg/L苯酚在氯过氧化物酶为10U/mL、pH为6.5、H2O2投加量为10mg/L时8h苯酚降解率分别达到86.6%和83.8%,比单纯菌株降解显著提高。说明氯过氧化物酶能快速清除苯酚污染的危害,提高苯酚的生物降解速率。

Chloroperoxidase（CPO） is a kind of chiral catalyst which has extensive catalytic activities for various substrates.It can be used for catalyzing the peroxidation of halogen ion,aromatic hydrocarbons,alcohols ect.In this paper,phenol was applied as study object to investigate the promotion effect of CPO on phenol biodegradation.The results show that the biodegradation of phenol was greatly improved with the existence of CPO.The optimal catalyzing conditions were CPO 10 U/mL,pH 6.5,H2O2 dosage of 10 mg/L and biodegrading for 8 h.When the initial concentration of phenol was 500,1 000 mg/L,biodegradation rate of phenol was 86.6% and 83.8% respectively under these optimal conditions.The conclusions indicated that CPO could quickly remove the phenol in environment and improve the phenol biodegradation efficiency significantly.