响应面试验优化超声波强化复合溶剂提取葡萄籽油工艺

Optimization of Ultrasonic-Assisted Extraction Using Composite Solvents of Grape Seed Oil by Response Surface Methodology

以新疆吐鲁番赤霞珠葡萄籽为原料,采用超声波强化复合溶剂提取葡萄籽油工艺过程。通过试验确定了正己烷-石油醚(7:3,V/V)溶液为最佳溶剂。在正己烷-石油醚溶液为复合溶剂条件下,以超声温度、料液比、超声时间、超声功率等单因素试验为基础,采用响应面分析法(RSM)对超声波辅助混合溶剂提取葡萄籽油工艺进行优化,得到最佳工艺条件,即固液比1:12(g/mL)、时间35.5min、功率290W、温度51℃。在此条件下葡萄籽油提取率为19.19%,所得葡萄籽油的各项指标均优于溶剂法所得葡萄籽油。

Cabernet-Sauvignon grape seeds collected from Turpan in Xinjiang were used as the subjects to extract grape seed oil through ultrasonic-assisted extraction.A mixture of hexane and petroleum ether at a ratio of 7：3（V/V） was considered as the best extraction solvent.Single factor tests were used to explore solid-liquid ratio,ultrasonic extraction temperature,ultrasonic extraction power and ultrasonic extraction time.Response surface methodology（RSM） was applied to optimize the extraction conditions.A maximum extraction rate of grape seed oil was achieved.The optimal extraction conditions were solid-liquid ratio of 1：12（g/mL）,extraction duration of 35.5 min,ultrasonic power of 290 W,and extraction temperature of 51 ℃.Under the optimal extraction conditions,the extraction rate of grape seed oil was up to 19.19%,and an improvement in the measured physicochemical indexes was achieved compared with the grape seed oil from solvent extraction.