畜禽产品中脱氧雪腐镰刀菌烯醇和T-2毒素残留分析

Analysis of Residual Deoxynivalenol and T-2 Toxin in Livestock and Poultry Products

目的:分析市售畜禽产品中脱氧雪腐镰刀菌烯醇(DON)和T-2毒素的残留情况。方法:试样经乙腈提取、正己烷脱脂、HLB柱净化,用高效液相色谱-质谱(HPLC-MS/MS)测定,外标法定量。结果:所有受检猪背脊肌肉、猪肝、猪肾、鸡胸肉、鸡腿肉、鸡翅肉和肉制品样品中均未发现DON残留,猪背脊脂肪样品中DON残留阳性率为28.75%,最高残留量为0.4265μg/kg;所有受检猪肝、猪肾和肉制品样品中均未发现T-2毒素残留,猪背脊肌肉、猪背脊脂肪、鸡胸肉、鸡腿肉、鸡翅肉样品中T-2毒素残留阳性率范围为8.33%～57.14%,最高残留量范围为0.0704～0.4515μg/kg。结论:在部分受检畜禽样品中检测到了DON和T-2毒素痕量残留。

Objective： To analyze residual deoxynivalenol（DON） and T-2 toxin in products of livestock and poultry.Methods： Residual DON and T-2 toxin in samples were extracted with acetonitrile,defatted with n-hexane,cleaned up on an HLB cartridge,and analyzed by high performance liquid chromatography coupled with tandem mass spectrometry（HPLCMS/MS） and quantified by an external standard method.Results： Residual DON was not observed in all tested samples of pig dorsal muscle,pig liver,pig kidney,chicken breast,chicken thighs,chicken wings and meat products,but detected in 28.75% of pig back fat,and the maximum residue was up to 0.4265 μg/kg.No residual T-2 toxin was detected in all tested samples of pig liver,pig kidney and meat products,but detected in 8.33%–57.14% of pig dorsal muscle,pig back fat,chicken breast,chicken thighs and chicken wings,and the maximum contents were 0.0704–0.4515 μg/kg.Conclusion： Trace residues of DON and T-2 toxin can be detected in partial tested samples of livestock and poultry products.