仿刺参生殖腺营养成分分析

Nutrient Composition of Apostichopus japonicas Gonad

对仿刺参生殖腺进行卵和精的分类,并分别进行营养成分分析。利用显微镜对生殖腺进行卵和精分类,经真空冷冻干燥后,分别测定粗蛋白、粗脂肪、粗灰分、粗多糖、磷脂及矿质元素含量;对卵和精的氨基酸组成、脂肪酸组成进行分析。结果表明:海参卵、精都具有较高的粗蛋白含量,分别达到51.80g/100g和74.31g/100g;卵的粗脂肪含量为10.18g/100g,显著高于精中的含量(4.26g/100g),而其中磷脂所占比例分别为71.32g/100g和52.35g/100g;卵和精的粗多糖含量为干质量的26.98g/100g和9.93g/100g;仿刺参精中Mg、Fe的含量高于仿刺参卵,而仿刺参卵当中Ca、Zn的含量要显著高于仿刺参精。卵和精的氨基酸总量分别为354.6mg/g和550.89mg/g,其中必需氨基酸含量分别为180.03mg/g和257.25mg/g;卵和精的脂肪酸组成中不饱和脂肪酸分别为73.22g/100g和62.61g/100g,其中,EPA含量最高,分别为15.98g/100g和15.27g/100g。

Apostichopus japonica spawn and sperm were identified through microscopic observation for nutrient composition analysis.The contents of crude protein,crude fat,crude ash,crude polysaccharide,phospholipid and mineral elements in vacuum frozen samples were determined along with the amino acid composition and fatty acid profile.The results showed that both the spawn and sperm had high level of crude protein contents,accounting for 51.80 g/100 g and 74.31 g/100 g,respectively.The crude lipid content of the spawn（10.18 g/100 g） was significantly higher than that of the sperm（4.26 g/100 g）,and phospholipid content was 71.32 g/100 g and 52.35 g/100 g in the spawn and sperm,respectively.The content of crude polysaccharide in the spawn and sperm were 26.98 g/100 g and 9.93 g/100 g,respectively.The contents of Mg and Fe in the sperm were higher than in the spawn of Apostichopus japonica,while the contents of Ca and Zn in the spawn were significantly higher than in the sperm.The total amino acids of the spawn and sperm were 354.6 mg/g and 550.89 mg/g,with essential amino acids accounting for 180.03 mg/g and 257.25 mg/g,respectively.As for fatty acids in the spawn and sperm,the unsaturated fatty acid contents were 73.22 g/100 g and 62.61 g/100 g,respectively,among which EPA accounted for 15.98 g/100 g and 15.27 g/100 g,respectively.