实时荧光聚合酶链式反应检测食用大豆油中动物源成分

Detection of Animal-Origin Ingredients in Edible Vegetable Oil by Real-Time PCR

为了从植物油中检出动物成分,在前人对引物研究结果基础上,自行设计2条动物线粒体16S rRNA基因特异性探针,采用实时荧光聚合酶链式反应检测技术对5份分别掺有猪油、牛油、羊油、鸡油和鱼油的大豆油样本进行检测。结果表明:5份模拟掺假大豆油中的动物线粒体16S rRNA基因片段均可被特异性检出,说明鉴别方法可靠;该法能检测出大豆油中0.05%的动物油脂成分,是一种很敏感的检测方法。

In recent years,vegetable oil adulterated with animal-origin ingredients appeared in the markets.In this work,a method for the detection of animal-origin ingredients in edible soybean oil by real-time fluorescence PCR was established,and two primers for animal mitochondria 16S rRNA gene were designed on the basis of data reported in the literature.For 5 samples of soybean oil adulterated with porcine,beef,sheep,chicken and fish oil,respectively,the animal-origin mitochondria specific genes were sensitively detected with a detection limit of 0.05%.