高效液相色谱法同时测定蛹虫草子实体中腺苷和虫草素含量

Simultaneous Determination of Adenosine and Cordycepin in Cordyceps militaris (L.) by High Performance Lquid Chromatography

采用高效液相色谱法建立同时测定蛹虫草子实体中腺苷和虫草素含量的方法,并对样品提取条件进行优化。以超纯水-甲醇(84:16,V/V)为流动相,流速1.0mL/min,经色谱柱XBridgeTM C18(4.6mm×250mm,5μm)分离,柱温30℃,进样量10μL,紫外检测波长254nm。腺苷和虫草素的空白回收率分别为99.13%和99.18%,加标回收率分别为95.78%和94.35%。经单因素及正交试验,最终确定的优化提取条件为提取液为水、提取时间40min、提取温度30℃、超声功率700W、提取次数1次。样品前处理及分析方法操作简便、回收率高、重复性好。

In this paper,an HPLC method for determining the contents of adenosine and cordycepin in Cordyceps militaris（L.） is described.The optimum conditions for extraction of adenosine and cordycepin were determined as pure water,40 min,30 ℃,700 W and 1 for extraction solvent,time,temperature,ultrasonic power and extraction number,respectively.The separation column was an XBridgeTM C18（5 μm,4.6 mm × 250 mm）,and the column temperature was 30 ℃.The mobile phase was composed of pure water and methanol（84：16,V/V） at a flow rate of 1.0 mL/min.The sample loading volume was 10 μL.UV detection was performed at 254 nm.The average recoveries from spiked blank and real samples were 99.13% and 95.78% for adenosine,and 99.18% and 94.35% for cordycepin,respectively.This method is characteristics of simplicity,and high sensitivity and accuracy.