红树林木果楝属植物可培养内生真菌的多样性及其代谢产物抗肿瘤活性

Diversity and in vitro antitumor activity of endophytic fungi from mangrove plants Xylocarpus

从3种红树林木果楝属植物材料的枝、叶及果皮中经反复分离纯化获得24株真菌,采用rDNA-ITS序列分析技术对菌株进行了种属鉴定,结果表明它们分属于3纲9目11科14属;并发现不同种属、不同产地的木果楝属植物内生真菌在种群组成和数量上存在差异,其中拟茎点霉属Phomopsis、炭疽病菌属Colletotrichum在3种植物材料中均分离得到。采用MTT法对菌液乙酸乙酯提取物进行体外抗肿瘤活性评价,发现15株真菌菌液乙酸乙酯提取物表现出良好的体外抗肿瘤活性,值得进一步研究和开发。

A total of 24 biologically pure entophytic fungal strains were isolated from stems,leaves,and seed coats of Xylocarpus plants by repeated purification,and identified with Internal Transcribed Spacer（ITS） rDNA molecular method,which belonging to 14 genera,11 families,9 orders and 3 classes.There were differences in genus and species levels among three plant materials from different habitats and species,and it was found that the strains of Phomopsis and Colletotrichum existed in all three plant materials.In vitro assay of antitumor activity by MTT method revealed that the EtOAc extracts of 15 strains exhibited potent antitumor activity.These results suggest that it is of value for further investigation on the above fungal strains.