热毒宁注射液对细菌内毒素性脂多糖致热大鼠的解热作用研究

Study on antipyretic effect of Reduning injection on lipopolysaccharide-induced fever rats

该实验观察热毒宁注射液(RDN)对细菌内毒素性脂多糖(LPS)发热大鼠的解热作用及对中枢发热介质的影响。将大鼠随机分为空白对照组、模型组、安乃近组、热毒宁注射液高、低剂量组(12,6 mL.kg-1)。除对照组外,所有大鼠均腹腔注射脂多糖(LPS,80μg.kg-1)观察各组体温变化,并采用双抗体夹心ELSIA法测定发热高峰大鼠下丘脑中环磷酸腺苷(cAMP)含量和肺组织中髓过氧化物酶(MPO)含量。热毒宁注射液高剂量组能明显降低发热大鼠体温的升高程度,并且能够降低发热大鼠下丘脑中cAMP含量和肺组织中MPO含量。热毒宁注射液有明显的解热作用,其作用机制可能主要与减少下丘脑中cAMP含量和肺组织中MPO含量有关。

To observe the antipyretic effect of Reduning injection（RDN） on lipopolysaccharide（LPS）-induced fever rats and its impact on centric fever medium.Rats were randomly divided into the blank control group,the model group,the Metamizole group,and high and low-dose RDN groups.Except for the blank control group,all of the rats were injected intraperitoneally with LPS（80 μg·kg-1） to observe their body temperature changes.The double-antibody sandwich ELSIA method was adopted to determine cAMP content in hypothalamus and MPO in lung tissues of fever peak rats.The high-dose RDN group can obviously reduce the temperature rise in fever rats,and cAMP and MPO content in hypothalamus.RDN showed significant antipyretic effect,which may be related with the reduction of cAMP content in hypothalamus and MPO in lung tissues.