广东省江门市淡水产品中香港海鸥菌的检测

Detection of Laribacter hongkongsis in freshwater products in Jiangmen,Guangdong

目的了解广东省江门市淡水产品中香港海鸥菌污染的状况。方法于2010年7—8月共采集江门市3家农贸市场6个不同品种的淡水产品共计161份进行检测,检样接种改良头孢派酮MacConkey琼脂(CMA)平板培养分离可疑菌株,经革兰染色、生化实验、特异性片段PCR扩增进行香港海鸥菌的鉴定,并选取5株进行了16SrRNA基因序列测定和比对分析。结果 161份检样中共检出香港海鸥菌20株,总阳性率为8.05%(20/161),其中香港海鸥菌在草鱼的阳性率为30.1%(19/63),5只虎纹蛙中检出1只阳性。结论江门市淡水产品中存在香港海鸥菌的污染,草鱼和蛙类可能是污染的重要来源,应加强监测和预警。

Objective To investigate the contamination status of Laribacter hongkongsis in freshwater products in Jiangmen, Guangdong province. Methods A total of 161 freshwater product samples were collected from three food markets in Jiangmen during July-August 2010. Laribacter hongkongsis strains were isolated by using modified Mac- Lonkey Agar. The positive samples in biochemical tests were further identified by specified PCR. The 16S rRNA of 5 isolates were sequenced and analyzed. Results A total of 20 Laribacter hongkongsis strains were detected from the 161 samples. The overall positive rate was 8.05%. The positive rate was 30.1% in grass carp （19/63） and 1 of 5 tigrina frogs was positive. Conclusion The contamination of Laribacter hongkongensis was detected in freshwater products in Jiangmen, especially in grass carp and tigrina frog. To ensure the food safety, it is necessary for health department to strengthen the monitoring of Laribacter hongkongsis contamination in freshwater products.