鲍曼不动杆菌OXA-23基因和ISAba1基因检测和耐药性关系

Detection of blaoxa-23 and ISAba1 genes in Acinetobacter baumannii and their relationship with drug resistance

目的了解鲍曼不动杆菌的耐药现状及OXA-23型碳青霉烯酶介导的耐药性。方法收集临床分离的200株鲍曼不动杆菌,用K-B(Kirby-Bauer)法进行常见药物的敏感性实验;通过PCR(polymerase chain reaction)扩增、克隆测序等分析OXA-23基因及ISAba1基因。结果温州医学院附属第一医院分离的200株鲍曼不动杆菌除对头孢哌酮/舒巴坦、亚胺培南、多粘菌素等保持相对较高的敏感性,对其余常见抗生素的耐药率均已超过了80%;200株鲍曼不动杆菌中共检到155株携带OXA-23基因,168株携带ISAba1基因。结论温州医学院附属第一医院鲍曼不动杆菌耐药情况严重,大多带有OXA-23基因,该基因编码的碳青霉烯酶应为其耐药的主要原因,ISAba1常伴随OXA-23型出现。

Objective To understand the current status of drug resistance of Acinetobacter baumannii and the resistance induced by genome b/aoxa - 23. Methods The susceptibility of 200 strains of A. baumannii to common antibiotics were tested by Kirby-Bauer method, and polymerase chain reaction was conducted to detect carbapenemases-related genome blaoxa - 23 and the insertion sequence of ISAbal. Results The resistance rates of cefoperazone/sulbactam, imipenem and polymyxin were relatively low, but the resistant rates to other antibiotics were all above 80%. Among the 200 strains, 155 carded blaoxa-23 gene and 168 carded the insertion sequence of ISAbal. Conclusion The drug resistance of A. baumannii in our hospital was very serious, many A. baumannii strains carried blaoxa -23 gene, which would be responsible for their drug resistance, the insertion sequence of ISAbal was often associated with the presence of blaoxa- 23 gene.