实验性家兔脊髓空洞症发病机制研究

Comparison of serial MRI studies and histological findings of experimental syringomyelia in rabbits

目的 探讨实验性脊髓空洞症发病机制。方法 取中国白兔 2 4只 ,其中Kaolin组 (12只 )经皮枕大池穿刺注入Kaolin制作动物模型 ,生理盐水组和假手术组 (各 6只 )作对照。术后行Kaolin组MRI扫描并与光镜、电镜组织学对比。结果 2周时MRI发现上颈髓呈水肿、缺血性改变。 4～ 6周 ,90 %动物形成空洞。空洞随观察时间延长而逐渐增大、受累节段增多。组织学证实了MRI发现。

Objective To investigate the etiology of experimental syringomyelia.Methods Twenty four Chinese white rabbits were divided into three groups: Kaolin group 12 rabbits, physiological saline group 6 rabbits and shamed operation group 6 rabbits, 25% Kaolin solution was injected into the cisterna magna of animals in the Kaolin goup to produce syringomyelia models, and the other two groups were designed as controls. Series MR images were obtained to observe development of the syringomyelia. The results were compared with the findings of light and electronic microscopy. Results At 2 weeks after injection, MRI revealed ischemic and edematous changes in upper cervical cord in 9 among the surivived 10 animals. At 4～6 weeks, (8/9)90% animals in Kaolin group developed syringomyelia. Syringomyelia enlarged gradually and the number of involved segments increased with time. Histological results consisted with the MRI fingings. Conclusions Our results suggest that ischemia and edema in the upper cervical cord and disturbance of cerebrospinal fluid circulation induced by Kaolin granuloma may play important roles in the formation of syrinx or hydromyelia in rabbits.