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钙敏感受体在颈动脉平滑肌细胞增殖和迁移中的作用 被引量：4

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摘要 目的观察钙敏感受体(calcium-sensing receptor,CaSR)在大鼠颈动脉平滑肌细胞中的表达情况及在颈动脉平滑肌细胞增殖和迁移中的作用。方法通过Realtime-PCR和Western Blot检测颈动脉平滑肌细胞中CaSR的表达情况;通过MTT法和Transwell法观察CaSR对颈动脉平滑肌细胞增殖和迁移能力的影响;通过Western Blot检测CaSR对MMP-9、MMP-2和PCNA表达的影响。结果 RT-PCR和Western blot结果显示在颈动脉平滑肌细胞中有CaSR的表达;MTT法和Transwell法结果显示与正常对照组相比较,CaSR激动剂组颈动脉平滑肌细胞增殖和迁移能力明显增强(P<0.05),而CaSR抑制剂组颈动脉平滑肌细胞增殖和迁移能力明显降低(P<0.05);Western Blot检测发现与正常对照组相比较,CaSR激动剂组MMP-9、MMP-2和PCNA表达明显增强,而CaSR抑制剂组MMP-9、MMP-2和PCNA表达明显降低。结论颈动脉平滑肌细胞中有CaSR的表达,并且CaSR可以影响颈动脉平滑肌细胞增殖和迁移。
作者 李辛 贺成业 罗俊生 张凤香
机构地区 辽宁医学院附属第一医院心外科
出处 《解放军医学院学报》 CAS 2013年第8期883-885,共3页 Academic Journal of Chinese PLA Medical School
基金 辽宁省自然科学基金项目(20092190) 辽宁医学院青年科技启动基金项目(Y2012Z011) 辽宁医学院附属第一医院青年科技启动基金项目(FY2012-07)
关键词 钙敏感受体 颈动脉平滑肌细胞 迁移 增殖
分类号 R34 [医药卫生—基础医学]
  • 相关文献

参考文献12

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共引文献18

同被引文献35

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引证文献4

二级引证文献9

解放军医学院学报
2013年 第8期

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