止颤胶囊对帕金森大鼠血清中细胞因子IL-1β和IL-6水平的影响

Effects of shive-stopping capsule on the serum levels of cytokines IL-1β and IL-6 in rats with Parkinson

目的探讨止颤胶囊对帕金森病(PD)大鼠细胞因子IL-1β和IL-6表达的影响。方法采用立体定位法将6-羟多巴胺(6-hydroxy dopamine,6-OHDA)注入大鼠黑质部位,制备帕金森大鼠模型。实验分3组,包括正常对照组、模型对照组和止颤胶囊组。采用酶联免疫吸附试验(ELISA)法对实验大鼠血清中细胞因子IL-1β和IL-6进行分析。结果与正常对照组相比,止颤胶囊组血清中IL-1β、IL-6基因的表达差异无显著性。与PD模型对照组相比,止颤胶囊治疗组IL-1β和IL-6的表达均有降低。结论止颤胶囊对PD大鼠血液中IL-1β和IL-6的表达有下调作用,表明止颤胶囊对PD有一定的治疗作用。关键词:止颤胶囊;帕金森病;细胞因子;IL-1β;IL-6;

Objective To study the effect of tremor-stopping capsule on cytokines IL-1β, IL-6 in the pathogenesis ofrats with Parkinson disease（PD）. Methods Using the method of injecting 6 - hydroxydopamine （6-OHDA） ininto the substantia nigra pars of rat＇s brain located to get injured PD model in part. We select. The experiment deviced into 3 groups, normal SD rats as the experimental control group, and PD rats are divided into PD model control group and tremor-stopping capsule group. ELISA were used to detect the groups all the above to analyse the expressionlevels of IL-1β and IL-6. in serum of experimental rats. The results of each group were statistically analyzed by SPSS 11.5, the groups are compared with each other to determine the effects of tremor-stopping capsule on the expression of IL-1β and IL-6. Results At the beginning of experiment, IL-1β and IL-6 protein expression waslevels ware observed higher in the peripheral blood of PD model control group and tremor-stopping capsule group compared with the normal control subjects （P 〈 0.05）; The expressionthe contents oflL-1β and IL-6 in tremor-stopping capsule group become lower followed with the time （P 〈 0.05）, and closer to the normal control group; Those in PD model control group decreased slightly compared with those without drug treatment （P 〉 0.05）. Conclusions The expression level of IL-1βand IL-6 in the substantia nigra parsblood of SD＇s PD rats is increased caused by PD, and it is decreased of both oflL-1β and IL-6 in serum of rats who treated by the tremor-stopping capsule. It is indicated that the traditional Chinese medicine tremor-stopping capsule has some treatment on PD＇s rats