HPLC法测定滋补生发丸中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量

Determination of 2,3,5,4'-Tetrahydroxy-stilbene-2-O-β-D-glucoside in Zibu Shengfa pills

目的:建立滋补生发丸中2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量的测定方法。方法:采用HPLC法,用ZORBAXSB-C18(250mm×4.6mm,5μm)色谱柱,乙腈-水(20:80)为流动相,流速1.0ml/min,检测波长为320nm。结果:2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.0257～0.4112μg浓度范围内线性关系良好(γ=0.9992)。平均回收率为98.78%,RSD为0.84%(n=6)。结论:方法简便,结果准确,可用于滋补生发丸的定量。

Objective： To establish a method for determining 2, 3, 5, 4＇-Tetrahydroxy-stilbene-2-O-fl-D-glucoside in Zibu Shengfa pills. Methods： The HPLC method was used with ZORBAX SB-C18 column （250X4.6mm, 5μm）, and acetonitrile-Water （20：80） was used as mobile phase. Flow rate was 1.0ml/min, detection wavelength was 320nm. Results： 2, 3, 5, 4＇-Tetrahydroxy-stilbene-2-O-fl-D-glucoside in 0.0257～0.4112μg showed a good linear relationship 0,=0.9992）. The average recovery was 98.78%, and RSD was 0.84% （n=6）. Conclusion This method is simple, results are accurate, and can provide a scientic fotmdation for controlling the quality of Zibu Shengfa pills.