子宫内膜异位症患者腹腔冲洗液及血清CD133、ABCG2的表达

Expression of CD133 and ABCG2 in peritoneal fluid and serum in patients with endometriosis

目的探讨子宫内膜异位症(EMs)腹腔冲洗液、血清CD133、ABCG2水平与EMs发生发展的关系。方法选择2010年1月～2011年6月江苏省镇江市妇幼保健院收治的EMs患者38例为EMs组,选择同期其他妇科良性病患者22例为对照组。采用酶联免疫吸附试验(ELISA)检测两组腹腔冲洗液及血清中CD133、ABCG2水平并分析其相关性。结果①Ems组腹腔冲洗液CD133、ABCG2水平分别为(13.47±2.37)、(9.81±2.45)ng/L,高于对照组的(11.75±2.82)、(8.14±2.92)ng/L,差异均有统计学意义(P=0.008、0.013)。血清CD133、ABCG2水平分别为(1.39±0.32)、(5.22±1.49)ng/L,高于对照组的(1.13±0.29)、(4.06±1.89)ng/L,差异均有统计学意义(P=0.001、0.006)。②EMs组Ⅲ～Ⅳ期患者腹腔冲洗液CD133、ABCG2水平分别为(14.28±1.99)、(10.55±2.29)ng/L,高于Ⅰ～Ⅱ期患者的(12.35±2.44)、(8.79±2.36)ng/L,差异均有统计学意义(P=0.011、0.027);血清CD133、ABCG2水平分别为(1.53±0.31)、(5.83±1.39)ng/L,高于Ⅰ～Ⅱ期患者的(1.19±0.22)、(4.39±1.21)ng/L,差异均有统计学意义(P=0.001、0.002)。③Ems组腹腔冲洗液CD133、ABCG2水平与血清CD133、ABCG2水平呈明显正相关(r=0.579,P=0.000;r=0.703,P=0.000)。结论腹腔冲洗液、血清CD133、ABCG2升高可能与EMs发生发展有关,有望单独或联合成为EMs诊断的新指标。

Objective To evaluate the expression of CD133 and ABCG2 in peritoneal fluid and serum in patients with endometriosis（EMs）.Methods 38 women with EMs from January 2010 to June 2011 in Maternal and Child Hospital of Zhenjiang City were selected as EMs group,22 women with other benign disease of gynaecology and without EMs were chosen as control group.Expression of CD133 and ABCG2 in peritoneal fluid and serum were detected by ELISA,the correlation was analyzed.Results ①The levels of CD133 and ABCG2 in the peritoneal fluid of EMs group were（13.47±2.37）,（9.81±2.45） ng/L and they were obviously higher than those of control group [（11.75 ±2.82）,（8.14± 2.92） ng/L],the differences were statistically significant（P = 0.008,0.013）.The levels of CD133 and ABCG2 in the serum of EMs group were（1.39±0.32）,（5.22±1.49） ng/L and they were obviously higher than those of control group [（1.13±0.29）,（4.06±1.89） ng/L],the differences were statistically significant（P = 0.001,0.006）.②The levels of CD133 and ABCG2 in the peritoneal fluid of the patients with stage Ⅲ-Ⅳ in EMs group were（14.28±1.99）,（10.55±2.29） ng/L and they were obviously higher than those of the patients with stage Ⅰ-Ⅱ in EMs group [（12.35±2.44）,（8.79±2.36） ng/L],the differences were statistically significant（P = 0.011,0.027）.The levels of CD133 and ABCG2 in the serum of the patients with stage Ⅲ-Ⅳ in EMs group were（1.53±0.31）,（5.83±1.39）,and they were obviously higher than those of the patients with stage Ⅰ-Ⅱ in EMs group [（1.19±0.22）,（4.39±1.21） ng/L],the differences were statistically significant（P = 0.001,0.002）.③CD133 and ABCG2 levels in serum were found had positive correlation with those in peri toneal fluid（r = 0.579,P = 0.000;r = 0.703,P = 0.000） in Ems group.Conclusion The rise of CD133,ABCG2 in peritoneal fluid and serum may have correlation with the pathogenesis of Ems,it may be a new index in the diagnosis of Ems independently or in combination with other index.