摘要
铜绿微囊藻菌ＣＣＡＰ１４５０／４在室内人工培养的结果表明，较大的无机培养液表面、连续适当光照２０ｕＥ／（ｍ２·ｓ）及适宜的温度（２５℃）有利于该藻菌的生产．通过超声波击碎细胞，离心过滤Ｃ１８净化柱分离，得到藻毒素．应用高效液相色谱分离主峰纯化藻毒素藻毒素在紫外光谱２３８ｎｍ处出现吸收高峰表明，铜绿微囊藻菌ＣＣＡＰ１４５０／４中藻毒素的主要成分是藻毒素ＬＲ．本研究还探索了简便、快速人工培养铜绿微囊藻菌及高效、叶靠分离纯化藻毒素ＬＲ的方法．
Microcystis aeruginosa CCAP 1450/4 was artificially cultured in a hasal medium. Results showed that greater surface area of medium, continuous suitable light 20uE/(m2.s) and optimum temperature (25C)enhanced the cell production. M. aeruginosa cell was broken up by ultrasonication. The microcystin was obtained by centrifugation filtration and C18 purification column extration. HPLC method was used to purify the microcystin. In the UV spectrum analysis, the microcystin showed a peak of absorbance at 238 nm. It indicated that major component of microcystin from M. aeruginosa CCAP 1450/4 was microcystin LR. An efficient, simple and reliable total system for M. aeruginosa cell artificial culture and microcystin LR extraction purification was explored.
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2000年第6期544-546,共3页
China Environmental Science
基金
国家留学基金委员会资助项目(97814035)
山西省自然科学基金资助项目(20001087)
关键词
铜绿微囊藻菌
细胞培养
藻毒素LR
分离纯化
Microcystis aeruginosa
cell culture
microcystin-LR
extraction purification
