雷公藤甲素对哮喘豚鼠肺组织表达IL-5、GM-CSFmRNA的作用

Action of Triptolide in the Expressions of IL-5,GM-CSF mRNA in Pulmonary Tissue of Asthmatic Guinea Pig

为探讨白介素 - 5(IL - 5)、粒细胞 -巨噬细胞集落刺激因子 (GM -CSF)mRNA在哮喘豚鼠肺组织中的表达及雷公藤甲素的影响。本组采用原位杂交方法检测 3组豚鼠 (共 30只 )肺组织IL - 5、GM -CSFmR NA的表达。结果发现哮喘组肺组织IL - 5、GM -CSFmRNA的表达强度分别为 (36 38± 7 6 5和 32 78±9 4 3) ;处理组分别为 (17 4 6± 7 32和 18 92± 6 38) ;对照组分别为 (13 82± 5 4 3和 18 2 7± 7 4 5)。哮喘组IL- 5、GM -CSFmRNA表达强度显著高于对照组和处理组 (P <0 0 1)。说明哮喘豚鼠肺组织IL - 5、GM -CSFmRNA高表达可能在哮喘气道炎症中发挥重要作用。而雷公藤甲素可能通过抑制IL - 5、GM

To research the expressions of IL-5,granulocyte macrophage colony stimulating factor(GM-CSF) in the asthmatic guinea pig pulmonary tissue and influence of triptolide on the expression,30 guinea pigs were divided at random into three groups:the asthma group(n=10),treated group(n=10) and control group(n=10).The expression levels of the IL-5,GM-CSF mRNA were detected by using in situ hybridization method.The results showed that the expressive intensities of IL-5,GM-CSF mRNA in asthma group were 36 38±7 65 and 32 78±9 43 respectively;the treated group were 17 46±7 32 and 18 92±6 38 respectively;control group were 13 82±5 43 and 18 27±7 45 respectively.The expressive intensities of IL-5,GM-CFS mRNA in asthmatic group were obviously higther than those of both treated and control groups,there was significantly difference between two groups(P<0 01).The data suggest that the overexpression of IL-5,CM-CSF in the pulmonary tissue of asthmatic guinea pig may be play an important role in development of the asthmatic airway inflammation.