GE7导入系统介导抑癌基因NOEY2体内导入治疗人上皮性卵巢癌裸鼠网膜移植瘤的研究

GE7-transferring-system-mediated NOEY2 gene therapy for human ovarian epithelial carcinoma transplanted in omentum of nude mice.

目的 :研究GE7导入系统体内导入效率及介导抑癌基因NOEY2体内导入后对人上皮性卵巢癌裸鼠网膜移植瘤的治疗作用。方法 :将人上皮性卵巢癌细胞株Hey细胞种植于裸鼠皮下成瘤后半包埋缝于裸鼠脾区网膜建立网膜移植瘤模型。将GE7- β -gal四元复合体经腹腔注入荷瘤鼠体内用X -gal染色法检测基因导入效率 ,同法导入生理盐水、GE7-pcDNA3四元复合体及GE7-pcDNA3-NOEY2四元复合体 ,研究其抑制肿瘤生长的作用。结果 :人上皮性卵巢癌裸鼠网膜移植瘤的成瘤率达 10 0 % ,3周后瘤体达 3 68± 0 82g。GE7- β -gal四元复合体经腹腔注入荷瘤鼠体内 12h后 β -gal即有表达 ,4 8h后以瘤体、肝、脾表达率最高。GE7-pcDNA3-NOEY2四元复合体腹腔注射 3周后瘤体生长抑制率为 4 0 81% (P <0 0 5)。结论 :人上皮性卵巢癌裸鼠网膜移植瘤模型具有实用性。GE7导入系统体内导入基因效率高 ,具有相对肿瘤靶向性。GE7导入系统介导抑癌基因NOEY2体内导入后能有效抑制上皮性卵巢癌的生长。

Objective:To study the efficiency of GE7-system-mediated NOEY2 gene therapy for human ovarian cancer transplanted in omentum of nude mice.Methods:The model was established by transplanting the subcutaneous tumor into omentum of nude mice.The transferring of β-gal gene and NOEY2 gene into peritoneum was introduced by using GE7-system to assay the efficiency of gene transferring and the tumor growth inhibitation.Results:All of the tumors grew gradually to 3.68±0.82g in 3weeks.β-gal was expressed in 12h after transferring and expressed strongly in tumor,liver and spleen in 48h.The tumor growth was inhibited by transferring NOEY2 gene (P<0.05).Conclusions:This animal model can be used practically.The GE7-system is a high-efficiencial and tumor targeting gene transferring vector.This gene therapy can inhibit the growth of ovarian cancer.