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MicroRNA-181b提高U87细胞对VM-26的敏感性研究 被引量:1

miR-181b Enhances Sensitivity to Teniposide in Glioma Cell Line U87
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摘要 背景与目的:MicroRNA(miRNA)参与肿瘤发生发展的诸多过程,并参与调节多种抗肿瘤药物的敏感性。本研究探讨恶性胶质瘤中miR-181b对VM-26(teniposide)化疗敏感性的影响。方法:以荧光定量PCR法检测miR-181b在高级别胶质瘤中的表达,并利用CCK-8细胞毒性实验检测高级别胶质瘤患者细胞对VM-26的化疗敏感性;并通过慢病毒感染构建稳定高表达miR-181b的U87/181b细胞及其对照组U87/nc,在荧光显微镜下观察其转染率及荧光定量PCR法检测其中miR-181b的表达;进而利用CCK-8细胞毒性实验检测U87/181b和U87/nc细胞对VM-26的敏感性,利用流式细胞仪检测VM-26作用72小时后U87/181b和U87/nc的凋亡情况。结果:在高级别胶质瘤中,miR-181b的表达与VM-26的敏感性呈正相关(r=-0.691,P﹤0.01),也就是miR-181b高表达肿瘤对VM-26的敏感性高。qPCR检测miR-181b在U87/181b(0.699±0.023)的表达显著高于U87/nc(0.019±0.001)(P﹤0.05)。CCK-8检测结果显示U87/181b[IC50:(1.25±0.12)μg/mL]对VM-26的敏感性显著高于U87/nc[IC50:(6.24±0.88)μg/mL](P﹤0.05)。经VM-26处理后U87/181b凋亡率(69.41±0.77)明显高于U87/nc(37.93±2.90)(P﹤0.05)。结论:在高级别胶质瘤高表达miR-181b的肿瘤对VM-26的敏感性高;在胶质瘤细胞U87中增加miR-181b表达可以提高对VM-26的敏感性。 BACKGROUND & OBJECTIVE: miR-181b is low expressed in glioblastoma which is also related to chemotherapy resistance. In this study, we aimed to investigate whether miR-181b enhances sensitivity to the chemotherapeutic agent teniposide (VM-26) in gliomas. METHODS: The expression of miR-181b was measured by qRT-PCR in frozen human glioma specimens and CCK-8 was used to determine growth inhibiting effects of VM-26 on glioma specimens. U87 cells were transfeeted with miR-181b (U87/181b) or control miRNA (U87/nc) by using lentiviriral system. And then, the expression level of miR-181b was measured by qRT-PCR and cell viability was detected by CCK-8 assay. Apoptosis was detected by flow eytometry. RESULTS: In high grade human glioma with higher miR-181b expression revealed more sensitive to VM-26, with significantly positive correlation (r=-0.691, P 〈 0.01 ). MiR-181b expression in U87/181b(0.699 ± 0.023)is significantly higher than in U87/ne(0.019±0.001 ) (P 〈 0.05). CCK-8 assay showed that U87/181b (IC50: 1.25±0.12ug/ml)was more sensitive to VM-26 than U87/nc (ICS0: 6.24±0.88ug/ml)(P 〈 0.05). U87/181b (69.41±0.77)had a higher apoptosis rate than U87/nc (37.93±2.90)(P 〈 0.05). CONCLUSION: miR-181b expression level is positively correlated to VM-26 sensitivity in high grade gliomas. In glioma cell line U87, enhancing miR-181b expression may increase its sensitivity to VM-26.
出处 《中国神经肿瘤杂志》 2013年第2期101-107,共7页 Chinese Journal of Neuro-Oncology
基金 863计划(中国人群脑胶质瘤分子分型与生物标志物研究) 广东省科学技术计划项目(No.2011B031800178) "中山大学青年教师培育计划"(赛克) 卫生部医药卫生科技发展研究中心项目(No.W2012FZ101)
关键词 胶质瘤 miR-181b VM-26 耐药性 Glioma miR-181b VM-26 Drug resistence
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  • 1Valencia CA, Cotten SW and Liu R. Cleavage of BNIP-2 and BNIP-XL by caspases. Biochem Biophys Res Commun 2007, 364: 495-501.
  • 2Longley DB, Allen WL and Johnston PG. Drug resistance, predictive markers and pharmacogenomics in colorectal cancer, t3iochim Biophys Acta 2006, 1766: 184-196.
  • 3Fojo T. Multiple paths to a drug resistance phenotype; mutations, translocations, deletions and amplification of coding genes or promoter regions, epigenetic changes and rniRNAs. Drug Resist Update 2007, 10: 59-67.
  • 4Allen WL, Coyle VM and Johnston PG. Predicting the outcome of chemotherapy for colorectal cancer. Curr Opin Pharmacol 2006, 6: 332-336.
  • 5Blower PE, Chung JH, Verducci JS, Lin S, Park JK, Dai Z and Liu CG, et al. MiRNAs modulate the chemosensitivity of tumor ceils. Mol Cancer Ther 2008. 7: 1-9.
  • 6Calin GA and Croce CM. MiRNA signatures in human cancers. Nat Rev Cancer 2006, 6: 857-866.
  • 7Bartel DP. MiRNAs: genomics, biogenesis, mechanism, and function. Cell 2004, 116: 281-297.
  • 8He L, Thomson JM, Hemann MT, Hemando-Monge E, Mu D, Goodson S and Powers S, et al. A miRNA polycistron as a potential human oncogene. Nature 2005, 435: 828-833.
  • 9Chen CZ, Li L, Lodish HF and Bartel DP. MiRNAs modulate hematopoietic lineage differentiation. Science 2004, 303: 83-86.
  • 10Chan JA, Krichevsky AM and Kosik KS. MiRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 2005, 65: 6029-6033.

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  • 1Zhao JJ,Lin J,Lwin T, et al. mieroRNA expression profile and identification of miR-29 as a prognostic marker and pathogenetie factor by targeting CDK6 in mantle cell. lymphoma[J]. Blood, 2010,115 (13) : 2630-2639.
  • 2Xin C,Buhe B, Hongting L, et al. MicroRNA-15a Promotes neuroblastoma migration by Targeting reversion-inducing cysteine-rich protein with Kazal motifs (RECK) and regulating MMP-9 expression[J].FEBS Letter, 2013,280(3) : 855-866.
  • 3Chen X,Pan M,Han L, et al. miR-338-3p suppresses neuroblastoma proliferation, invasion and migration through targeting PREX2a[J]. FEBS Lett, 2013,587 : 3729-3737.
  • 4Wu L,CaiC,Wang X, et al. MicroRNA-142-3p, a new regulator of RAC1, suppresses the migration and invasion of hepatocellular carcinoma cells[J]. FEBS letters, 2011, 585 (9) : 1322-1330.
  • 5MacKenzie TN, Mujumdar N, Banerjee S, et al. Triptolide induces the expression of miR-142-3p: a negative regulator of heat shock protein 70 and pancreatic cancer cell proliferation [J]. Mol Cancer Ther,2013, 12(7) : 1266-1275.
  • 6李勤,汪洋,金先庆,刘伟,赵珍珍.超声辐照对神经母细胞瘤株化疗敏感性的影响[J].中华小儿外科杂志,2008,29(8):467-470. 被引量:3
  • 7陈传贵,于振涛.抗肿瘤治疗的新靶点—HMGB1[J].中国肿瘤临床,2009,36(2):114-117. 被引量:2
  • 8程文,高建平,张征宇,葛京平,徐锋,位志峰.Ⅱ级膀胱尿路上皮癌microRNA差异表达及意义[J].医学研究生学报,2010,23(1):48-52. 被引量:20
  • 9吴芳,李刚,段玉霞,吴建胜,梁瑛琦.miRNA在有无淋巴结转移结肠癌中的表达[J].肿瘤学杂志,2011,17(12):931-934. 被引量:3
  • 10魏晓萍,惠起源.HMGB1与肿瘤的相关性[J].现代肿瘤医学,2013,21(7):1639-1642. 被引量:3

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