饮酒、高脂饮食、疲劳对男性健康志愿者谷丙转氨酶、谷草转氨酶影响随机平行对照研究

Random Parallel Controlled Study about The Influence of Alcohol,High Fat Diet,Fatigue on Alanine Aminotransferase,Aspartate Aminotransferase in Healthy Male Volunteers

[目的]观察饮酒、高脂饮食、疲劳对谷丙转氨酶、谷草转氨酶影响。[方法]使用随机平行对照方法,将24例男性健康志愿者按随机数字表分为四组,饮酒组、高脂饮食组、剧烈运动组、空白组,6人/组。观察-3~0d筛选男性健康志愿者,签署知情同意书,相关指标基线检测;1d三餐统一饮食控制（清淡素食）。2d07：00空腹采静脉血3mL,三餐统一饮食控制（清淡素食）。3d07：00空腹采静脉血3mL;早餐、午餐统一饮食控制（清淡素食）;16：30疲劳组：剧烈运动,16：50采静脉血3mL;17：00四个观察组同时进晚餐;晚餐后4h采集静脉血3mL;禁食12h。4d07：00空腹采静脉血3mL,试验结束。观测谷丙转氨酶、谷草转氨酶值。出组后三餐进普通饮食,随访1周观测肝功能指标（ALT、AST）。[结果]ALT饮酒后4h较基线平均升高0.6 u/L,饮酒次日较基线平均升高2.0u/L;ALT在高脂饮食后4h较基线平均升高0.83 u/L,高脂饮食次日较基线平均升高0.50u/l;ALT在剧烈运动后较基线平均升高0.5 0u/L,剧烈运动餐后4h无变化,剧烈运动次日较基线平均降低0.33u/L。AST饮酒后4h较基线平均升高3.0u/L,饮酒次日较基线平均升高0.8u/L。AST在高脂饮食后4h较基线平均升高1.00u/L,高脂饮食次日较基线升高1.17u/L。AST在剧烈运动后较基线平均升高3.83u/L,剧烈运动餐后4小时升高0.83u/L,剧烈运动次日较基线平均升高2.33u/L。[结论]饮酒、高脂饮食、疲劳（剧烈运动所致）均有提高血中ALT含量趋势,饮酒作用相对明显,但三者对肝功检测数据影响变化范围较小,且在正常值范围内。

[ Objective ] To observe the influence of alcohol, high fat diet, fatigue on alanine aminotransferase, aspartate aminotransferase. [ Method ] Using randomized controlled methods, 24 healthy male volunteers were randomly divided into four groups, drinking group, high fat diet group, fatigue group and control group, 6 person / group. Observation of -3 ~ 0day screening healthy male volunteers, signed the informed consent, correlation index baseline detection; 1st d unified control （ light vegetarian ） . 2nd d 07 ： 00 collected venous blood 3mL on an empty stomach , three meals a day diet control （ light vegetarian ） . 3rd d 07 ： 00 fasting venous blood 3mL ;breakfast, lunch unified diet control （light vegetarian ） ; 16 ： 30 fatigue group： strenuous exercise fatigue, 16 ： 50 collected venous blood 3mL; 17 ： 00 four observation groups had supper ; after supper 4 hours collected venous blood 3mL; fasting 12h. 4th d 07 ： 00 fasting venous blood 3mL at the end of the test. Observed alanine aminotransferase, aspartate aminotransferase values. If the detection value anomaly at the end of the test, review sequence detection according to 3rd, 7th day time, follow-up to the normal, Steady level or the level before treatment. [ Results ] ALT increased by an average of 0.6 u/L 4h after drinking, the next day increase 2.0u/L compared to the baseline ; ALT increased by an average of 0.83u/L after having high fat diet 4h compared to the baseline, the next day increase 0.50u/1 compared to the baseline. ALT increased byan average of 0.50u/L after vigorous exercise compared to the baseline, had no change after a meal 4h. ALT was reduced 0.33u/L in strenuous exercise group the next day compared to the baseline. AST increased by an average of 3.0 u/L 4h after drinking, the next day increase0.8u/L compared to the baseline; AST increased by an average of 1.00u/L after having high fat diet 4h compared to the baseline, the next day increasel. 17u/1 compared to the baseline. AST increased by an average of 3.83u/L after vigorous exercise compared to the baseline, increased 0.83u/L after meal 4h. ALT increased 2.33u/L in strenuous exercise group the next day compared to the baseline. [ Conclusion ] Alcohol, high fat diet, fatigue （ caused by strenuous exercise ） had increased serum ALT level, alcohol consumption effect was relatively obvious, but the three effects on liver function values range were small and within normal range.