增殖性玻璃体视网膜病变玻璃体血管内皮生长因子表达的研究

Study of the vascular endothelial growth factor express in vitreous of proliferative vitreoretinopathy

目的 研究血管内皮生长因子 (VEGF)在增殖性玻璃体视网膜病变 (PVR)玻璃体中的表达 ,分析VEGF在 PVR中的作用。方法 增殖性玻璃体视网膜病变 37例 ,其中视网膜脱离 PVR2 2例 2 2眼 ,外伤性 PVR15例。以 12例猝死无眼疾的成年人作为正常对照。检测病例组、对照组玻璃体 VEGF水平。VEGF的测定采用对抗夹心法 (EL ISA)。结果 37例增殖性玻璃体视网膜病变玻璃体 VEGF平均 2 2 0 .5 2± 10 1.97pg/ m l,其中 2 2例网脱PVR玻璃体 VEGF2 38.90± 6 1.2 4pg/ ml,外伤性 PVR玻璃体 VEGF193.5 8± 5 8.17pg/ ml。正常对照组玻璃体VEGF89.90± 36 .36 pg/ ml。PVR玻璃体 VEGF水平明显高于正常对照组 (P <0 .0 5 )。VEGF在 PVRA级、B级水平较高 ,且随 PVR程度增加而降低。有危险因素 PVR,VEGF水平高于无危险因素 PVR(P <0 .0 5 )。结论 作为细胞间的传导信号 ,VEGF参与 PVR的发生发展。VEGF在 PVR中呈上调性表达。VEGF不仅在缺血性眼部疾病中呈高表达 ,而且在某些非缺血性眼部疾病中表达升高。 VEGF主要在 PVR早期发挥作用 ,PVR危险因素可以刺激玻璃体 VEGF上行性表达。

Objective To investigate the expression of vascular endothelial growth factor (VEGF) in vitreous of proliferative vitreoretinopathy (PVR),dertermine the function of VEGF in prolferative vitreoretinopathy.Methods The level of vitreous'VEGF were serially measured by ELIA method in 37 proliferative vitreoretinopathy.8 ischemical eye diseases and 12 normal contrast.Results The cytokine level in vitreous of 32 PVR:VEGF is 220.52±101.97pg/ml.In which,retinal detachment PVR VEGF leve is 238.90±61.24pg/ml;traumatic PVR VEGF is 193.58±58.17pg/ml/Ischemical eye diseases VEGF is 850.50±301.40pg/ml.Normal contrast VEGF is 89.90±36.46pg/ml.The level of VEGF in vitreous of PVR is significantly higher than normal contrast (P<0 05).VEGF in eyes with grade A B PVR tend to be higher than eyes with grade C、D PVR Otherwise,VEGF tends to decrease with PVR grade increase.Conclusion As the soluble conducate signals between cells,cytokines VEGF have a role in occurrence and development of proliferative vitreoretinopathy.Not only has VEGF a high expression in ischemical eye disease,but also significantly elevate in some nuischemical eye disease.VEGF may play a main role in the earlier stages of PVR.