摘要
目的以高效液相色谱法(HPLC)测定清肝败毒丸中姜黄素含量。方法色谱柱:Phenomenex Synergi C18(250mm×4.6mm,4μm),流动相:乙腈-4%冰醋酸溶液(48∶52),检测波长:430nm,流速:1.0ml/min。结果姜黄素在0.03～0.27μg范围内线性关系良好,r=0.9999,平均加样回收率为110.9%,RSD=1.5%(n=6)。结论 HPLC操作简便,结果准确可靠,可作为清肝败毒丸中姜黄素的含量测定方法。
Objective To establish a HPLC method for determination of curcumin in Qinggan Baidu pills.Methods Analytical Column was Phenomenex Synergi C18（250mm×4.6mm,4μm）,the mobile phase was acetonitrile-4% acetic acid solution （48∶52）,the UV detection wavelength was 430nm,the flow rate was 1.0ml/min.Results The linear range of curcumin were at 0.03μg～0.27μg （r=0.9999）,The average recovery （n=6）were 110.9% ,with RSD 1.5% .Conclusion The method of HPLC is simple,available and accurate,which can be used to determining the content of curcumin in Qinggan Baidu pills.
出处
《临床合理用药杂志》
2013年第23期33-34,共2页
Chinese Journal of Clinical Rational Drug Use
