血红素加氧酶-1重组乳酸乳球菌对失血性休克大鼠肠黏膜屏障和血气分析的影响

Effects of L.Lactis recombinant heme oxygenase-1 gene on the intestinal barrier and blood-gas analysis in rats with hemorrhagic shock

目的 研究失血性休克大鼠股动脉血气分析和平均动脉压（MAP）的变化情况,进一步探索血红素加氧酶-1（HO-1）的保护机制.方法 将20只健康、清洁级、雄性SD大鼠复制失血性休克模型,随机分为：HO-1重组乳酸乳球菌灌胃组（HO组）和磷酸盐缓冲液（PBS）灌胃组（PBS组）.比较两组血气分析、MAP,液体复苏后1h死亡率、细菌移位、髓过氧化物酶（MPO）活性及HO-1、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的含量,并行肠组织病理学检查.结果 与PBS组比较,HO组的死亡率、Chiu＇s评分、细菌移位发生率、MPO活性明显降低[10％与40％、（1.51±0.23）分与（2.15±0.48）分、44.4％与100.0％、（0.16±0.05）U/g与（0.99±0.28） U/g,均P＜0.05]；复苏后的PaO2、MAP、HO-1含量和IL-10灰度值明显增加（均P ＜0.05）.结论 该实验条件下,HO-1重组乳酸乳球菌在失血性休克期能够维持较高的PaO2和MAP,利于肠黏膜屏障保护和减轻肠道炎性反应.

Objective To study the protection mechanism of HO-1 with the gas-blood analysis and MAP of femoral artery in rats with hemorrhagic shock. Methods A model of hemorrhagic shock was established in 20 SD healthy clean male rats. The rats were randomly divided into the L. Lactis recombinant HO-1 gene group （HO group） and phosphate buffer soLution group （ PBS group）. The gas-blood analysis and MAP of femoral artery in hemorrhagic shock were compared during the test. The mortality rate, MPO activity,bacterial translocation,the pathologic and con- tent of HO-1 ,TNF-α and IL-10 in the low intestine were detected and compared lh after resuscitation. Results Com- pared to PBS group, the mortality rate, Chiu＇s grade, bacterial translocation and MPO activity in HO group were signif- icantly decreased [ 10% vs 40%, （ 1.51 ± 0.23 ） points vs （2.15 ± 0.48） points,44.4% vs 100.0%, （0.16 ± 0.05 ） U/g vs （0.99 ± 0.28） U/g, all P 〈 0.05 ], PaO2 and MAP during the resuscitation, the content of HO-1 and the gray level of IL-10 were significantly increased. Conclusion L Lactis recombinant HO-1 gene has the virtue to maintain the higher level of PaO2 and MAP,which is beneficial to the intestine mucosa barrier and anti-inflammation response of low intestine significantly.