金莲花滴丸中荭草苷和牡荆苷的含量测定方法探讨

Determination of orientin and vitexin in Jinlianhua Pills by HPLC

目的 探讨金莲花滴丸中荭草苷和牡荆苷的含量测定方法.方法 采用高效液相色谱法测定荭草苷和牡荆苷的含量,以Shimadzu VP-ODS（250×4.6 mm,5μm）色谱柱,0.5％冰醋酸水溶液-乙腈（88：12）为流动相,检测波长为340 nm,柱温35℃.结果 荭草苷在25.0～75.0μg/ml范围内呈良好的线性关系,平均回收率为99.9％,RSD为1.2％；牡荆苷在8.0～24.0μg/ml范围内呈良好的线性关系,平均回收率为100.5％,RSD为1.3％.结论 采用高效液相色谱法测定荭草苷和牡荆苷的含量准确可靠,重复性好,可作为金莲花滴丸的质量控制方法.

Objective To develop a HPLC method for quantitative determination of orientin and vitexin in Jinlianhua Pills and to provide valuable data for quality control of Jinlianhua Pills.Methods The separation was performed on a Shimadzu VP-ODS column （250 x 4.6mm,5μm）at 35℃ using a mobile phase of 0.5% acetic acid solution-acetonitrile（88：12）.The detection wavelength was 340nm.Results The calibration curve of orientin showed a good linearly within test ranges of 25.0 ～ 75.0μg/ml.The average recovery was 99.9% with RSD 1.2%.The calibration curve of vitexin showed a good linearly within test ranges of 8.0 ～ 24.0μg/ml.The average recovery was 100.5% with RSD 1.3%.Conclusion The developed method is simple,accurate and with good repeatability.It is suitable for quality evaluation of Jinlianhua Pills.