肺泡灌洗液肺炎支原体DNA检测对儿童支原体肺炎的诊断价值

Detection and analysis of MP-DNA in BALF of mycoplasma pneumoniae pneumonia in children

目的 探讨支气管肺泡灌洗液（BALF）支原体DNA检测对支原体肺炎的早期诊断价值.方法 对103例肺炎患儿行BALF支原体DNA定量检测,同时对患儿的血清行支原体IgM定性检测,并以55例支气管异物患儿作为对照组.结果 肺炎患儿BALF支原体DNA阳性率为32.43％,血清支原体IgM抗体阳性率为19.43％,两种支原体阳性率明显高于对照组（x2=13.00、4.11,均P＜0.05）；且BALF支原体DNA阳性率明显高于血清支原体IgM阳性率（x2=4.92,P＜0.05）.结论 BALF支原体DNA定量检测较传统血清支原体IgM定性检测而言,标本的留取相应难度较大,但可避免血清IgM空窗期结果的假阴性,对MPP患儿有更高的诊断价值.

Objective To study the value of MP-DNA in the early diagnosis of pneumonia by analyzing the result of MP-DNA in bronchoalveolar lavage fluid（BALF） and MP-IgM antibodies in serum. Methods 103 hospital- ized children were detected for MP-DNA in BALF（ positive：DNA copies 〉 102/ml） and MP-IgM antibodies in serum. Results The MP-DNA positive in BALF and the MP-IgM positive in serum in children with pneumoina were signifi- cantly higher than those in children with foreign body in bronchus（ X2 = 13.00,4.11, all P 〈 0.05 ）. 33.01% of 103 children was MP-DNA positive, 19.43% of 103 children was MP-IgM positive. The MP-DNA positive in BALF was significantly higher than the MP-IgM positive in serum （ X2 = 4.92, P 〈 0.05 ）. Conclusion The samples of MP-DNA in BALF were collected more difficulty than the samples of MP-IgM in serum, but the detection of MP-DNA can avoid the false negative, which may be of value in the diagnosis and therapy of MPP.