Hp感染性胃病与T_H1/T_H2细胞因子行为的关系

Relationship of type T_H1/T_H2 cytokines in Hp infected gastric diseases

目的探讨 Hp 感染性胃部疾病患者 T_H1/T_H2型细胞因子的含量变化.方法对患不同胃部疾病的 Hp 阳性患者109例及 Hp 阴性患者30例的外周血单个核细胞(PBMC)进行离心、培养,取上清液后,对其 T_H1型细胞因子 IFN-γ,IL-2及 T_H2型细胞因子IL-4,IL-10的含量采取双抗体夹心 ELISA 法进行检测.结果在不同胃部疾病伴 Hp 感染者 IFN-γ,IL-2的含量分别较对照组明显增高,(CSG,PU,CAG,GC:IFN-γ:ng·L^(-1):55±21,64±15,99±32,195±39 vs 42±18;IL-2;ng·L^(-1):37±15,40±17,47±7,69±20 vs 29±16,t 检验,P<0.05).而 IL-4,IL-10的含量大部分无显著差异(CAG,GC:IL-4:ng·L^(-1):21±6,32±12 vs 35±22;CSG,PU,CAG:IL-10:ng·L^(-1):40±24,28±19,27±5 vs 29±21,t 检验,P>0.05),PU 患者 IL-10的含量明显低于 CSG 患者(ng·L^(-1):28±19 vs40±24,t 检验,P<0.05);CAG IFN-γ的含量明显高于 CSG和 PU(ng·L^(-1):99±32 vs 55±21,64±15,t 检验,P<0.001);GC 患者 IFN-γ,IL-2,IL-4,IL-10含量明显高于CAG 患者(ng·L^(-1):195±39,69±20,32±12,58±17 vs 99±32,47±7,21±6,27±5,t 检验,P<0.05～0.001).结论 Hp 感染性胃部疾病患者均存在细胞免疫功能亢进,表现为 T_H1优势应答.在不同的胃部疾病之间某些细胞因子含量存在差异.

AIM To study the level of type T_H1/T_H2 cytokines in Hp infected gastric disease patients. METHODS The level of IFN-γ,IL-2,IL-4,IL-10 in the supernatant of separation and culture of the PBMC were measured by ELISA respectively for the 109 patients with Hp infection and for the 30 patients without Hp infection. RESULTS The results showed that the levels of IFN-γ,IL- 2 in the different disease groups with Hp infection were higher significantly than those without Hp infection (CSG, PU,CAG,GC:IFN-γ:ng·L^1:55±21,64±15,99±32,195 ±39 vs 42±18;IL-2;ng·L^(-1):37±15,40±17,47±7,69±20 vs 29±16,t-test,P<0.05).The levels of IL-4,IL-10 in some patients with or without Hp were not different significantly (CAG,GC:IL-4:ng·L^(-1):21±6,32±12 vs 35 ±22;CSG,PU,CAG:IL-10:ng·L^(-1):40±24,28±19,27±5 vs 29±21,t-test,P>0.05).The contents of IL-10 in PU patients were much lower than in CSG (ng·L^(-1):28±19 vs 40±24,t-test,P<(0.05);The content of IFN-γ in CAG patients were higher than in CSG and PU (ng·L^(-1):99±32 vs 55±21,64±15,t-test,P<0.001);The contents of IFN-γ,IL-2,IL-4,IL-10 in patients of GC were higher than those of CAG(ng·L^(-1):195±39,69±20,32±12,58±17 vs 99±32,47±7,21±6,27±5,t-test,P<0.05-0.001). CONCLUSION Hp infected gastric disease patients have hyperfunction of cellular immunity.They all display a T_H1 predominant response.The levels of some cytokines in different gastric diseases varies.