食管鳞癌组织中Twist1的表达及其临床意义

Expression of Twist1 in esophageal squamous cell carcinoma tissues and its clinical significance

目的探讨转录因子Twist1基因在食管鳞癌组织中的表达及其临床病理学意义。方法采用免疫组化EnVision法检测Twist1基因在164例食管鳞癌中的表达,分析Twist1基因表达与患者年龄、性别、肿瘤大小、有无淋巴结及远处转移、肿瘤分化程度、临床分期及生存率等临床病理学特征之间的关系。结果 Twist1在食管鳞癌组织中主要表达于癌周边间质纤维细胞,部分表达于癌细胞。食管鳞癌间质细胞中Twist1在T1期和临床分期Ⅰ期中的表达明显低于其他分期(P均<0.05),有淋巴结转移组高于无淋巴结转移组(P<0.05),死亡组高于生存组(P<0.05);Twist1在食管鳞癌间质细胞中的表达与患者性别、年龄、肿瘤大小、分化程度、有无远处转移和肿瘤复发均无关(P>0.05)。食管鳞癌细胞中Twist1表达在死亡组明显高于生存组(P<0.001),复发组明显高于未复发组(P<0.001);Twist1在食管鳞癌细胞中表达与患者性别、年龄、肿瘤大小、分化程度、TNM分期均无关(P>0.05)。结论检测Twist1在食管鳞癌中的表达可成为判定食管鳞癌患者预后的重要辅助参考指标。

Purpose To investigate the clinicopathological significance of transcript factor Twistl gene expression in esophageal squamous cell carcinomas, mothods The expression of Twistl was detected by EnVision immunohistochemical method in 164 cases of esophageal squamous cell carcinoma tissues, and the relationship between Twistl expression and clinical and pathological features, such as patient age, sex, tumor size, lymph node and distant metastasis, differentiation, clinical stage and survival, was analyzed. Results Twistl was mainly expressed in stromal fibroblasts surrounding cancer cells, and some expressed in cancer cells in esophageal squamous cell carcinoma. The stromal fibroblast expression of Twistl in esophageal squamous cell carcinoma was lowest in stage T1 and TNM1 compared with other stages（ both P 〈 0. 05 ）, and the expression in lymph node metastasis group was higher than that without lymph node metastasis （P 〈 0. 05）, and in death group was higher than that of survival group （P 〈 0. 05）. However, there was no relationship between Twistl expression and patients＇ gender, age, tumor size, differentiation, distant metastasis and cancer recurrence （ P 〉 0.05 ）. The cancer cell expression of Twistl in esophageal squamous cell carcinoma was higher in death group than that in survival group （P 〈 0. 001 ）, and that in recurrence group was higher than no recurrence group （P 〈 0. 001 ）. Conclusion Twistl expression might be an important marker for detection of prognosis in patients with esophageal squamous cell carcinoma.