非酒精性脂肪性肝病大鼠血清白介素18、白介素10及其比值的变化和意义

Change of plasma interleukin-18,interleukin-10 and interleukin-18/interleukin-10 ratio in rats with non-alcoholic fatty liver disease

目的观察非酒精性脂肪性肝病(NAFLD)大鼠血清白介素(IL)-18、IL-10及其比值变化,探讨其在NAFLD发生中的意义。方法采用ELISA法检测20例正常大鼠和20例NAFLD模型大鼠血清IL-18、IL-10的水平并计算出IL-18/IL-10比值。结果 NAFLD模型组大鼠肝细胞脂肪变性、炎症较明显;血清IL-18水平、IL-18/IL-10比值明显高于正常组,而IL-10水平低于正常组(P<0.05),且IL-18水平和炎症程度相关(r=0.71,P<0.05),IL-10水平、IL-18/IL-10比值与炎症程度和脂肪变程度相关(r=-0.64,P<0.05;r=-0.46,P<0.05;r=0.70,P<0.05;r=0.61,P<0.05)。结论 NAFLD模型组大鼠IL-18水平与炎症程度呈正相关;IL-10水平与大鼠肝脏脂肪变性和炎症程度呈负相关,IL-18/IL-10比值与大鼠肝脏脂肪变性和炎症程度呈正相关,提示IL-18/IL-10比值可能对预测NAFLD的发生有一定意义。

Objective To investigate the change of interleukin-18（IL-18）,interleukin-10（IL-10） and IL-18/IL-10 ratio in rats with non-alcoholic fatty liver disease（NAFLD）,and inquire their roles in the development of NAFLD.Methods The serum levels of IL-18 and IL-10 in rats with NAFLD were detected by enzyme linked immunosorbent assay（ELISA） then calculating the IL-18/IL-10 ratio.Results The change of steatosis and inflammation were notable in NAFLD;the serum levels of IL-18 and IL-18/IL-10 ratio in NAFLD were higher than that of normal group（P0.05）,while IL-10 was lower than those in normal group（P0.05）;IL-18 in NAFLD was correlated with the development of inflammation（r=0.71,P0.05）,IL-10 and IL-18/IL-10 were correlated with the development of inflammation and steatosis（r=-0.64,P0.05;r=-0.46,P0.05;r=0.70,P0.05;r=0.61,P0.05）.Conclusion IL-18 is positively correlated with the development of inflammation in rats with NAFLD;IL-10 is negatively correlated with the development of inflammation and steatosis in NAFLD,while IL-18/IL-10 ratio is positively correlated.IL-18/IL-10 plays an important role in the development of NAFLD.