酒精对小鼠脑神经瘤细胞Neuro2a增殖的影响

Effects of cell proliferation in mouse brain tumor cells Neuro2a by alcohol

目的探讨酒精通过控制中心体增长影响小鼠脑神经瘤细胞Neuro2a增殖的机制。方法免疫荧光细胞计数检测酒精对细胞周期进程的改变;免疫荧光法检测酒精对纺锤体表型以及对外源性和内源性中心体蛋白P4.1相关蛋白(CPAP)中心体定位浓度的影响。结果 100 mmol/L酒精处理Neuro2a细胞96 h后,阻碍了细胞周期的进程,减少了进入有丝分裂期的细胞量;有丝分裂期纺锤体的表型出现多种异常;酒精通过降低中心体上内源性和外源性CPAP的定位浓度,抑制了中心体的增长。结论酒精抑制CPAP在中心体增长中的功能,导致有丝分裂期纺锤体排列异常,从而阻止细胞进入有丝分裂期,引起Neuro2a细胞数量减少。

Objective To investigate the mechanism of the effects of cell proliferation in mouse brain tumor cells Neuro2a treated by alcohol through centrosome elongation regulation. Methods The change of cell cycle treated by alcohol was detected by cell counting in immunofluorescence. The spindle phenotype and the amount of location of exogenous and endogenous centrosomal P4.1-associated protein （CPAP） in centrosome were observed by immuno- fluorescence. Results Number of cells into the mitotic phase was reduced by cell cycle arresting after treatment with 100 mmol alcohol in 96 hours. The abnormal phenotypes of spindles were observed in mitosis cells treated with alcohol. Alcohol inhibits the centrosome elongation by reducing the amount of location of exogenous and endogenous CPAP in centrosome. Conclusion Numbers of Neuro2a cells are reduced by abnormal spindle alignment and bloc-king cell into the mitotic phase through the inhibition of centrosome elongation by alcohol.