结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定

Separation of 5-fluorouracil,leucovorin calcium and oxaliplatin based on HPLC method

目的结直肠癌患者尿液中5-氟尿嘧啶、奥沙利铂和亚叶酸钙3种常用药物含量的测定。方法对4名结直肠癌患者尿样中5-氟尿嘧啶、奥沙利铂和亚叶酸钙3种药物残留量采用高效液相色谱法进行分离和分析，流速1mL／min，流动相，pH-3磷酸水溶液／甲醇（80／20v／v），检测波长270nm，色谱柱Inertsil ODS（150mm×4．6mm，5μm）。结果5-氟尿嘧啶、奥沙利铂和亚叶酸钙的检出限分别为42．0、294、33．3μg／L，线性范围1．0～1000．0mg／L，相对标准偏差（RSD）分别为0．34％、0．98％、1．00％，回收率93．40％～110．70％。结论该方法样品处理简便，色谱分离完全，结果准确可靠，为结直肠癌患者尿液中的药物代谢组学研究提供了基础。

Objective To investigate the detection of the amount of 5-fluorouracil, leucovorin calcium and oxaliplatin in the urine of colorectal cancer patients. Methods The urine of 4 colorectal cancer patients was used by HPLC to separate and analyse the three above-mentioned drugs with flow rate （1 mL/min）, mobile phase （phosphoric acid solution/methanol （80/20 v/v））, detection wavelength （270 nm） and column （Inertsil ODS （150 mm × 4.6 mm, 5 tam））. Results The detection limits of 5-fluorouracil, leucovorin calcium and oxaliplatin were 42.0, 29.4, 33.3 μg/L, respectively. The linear range was 1.0-1 000.0 mg/L. The relative standard deviation was 0.34%, 0.98%, 1.00%, and recovery rate was between 93.40 and 110.70%. Conclusion This method is simple, inertsil being fully separated, the result being reliable, which provides a base for future metabolomics research in the urine of patients with colorectal cancer.