乙肝病毒感染骨髓细胞的研究

Study of HBV infecting cells in bone marrow

为明确乙肝病毒对骨髓中三种细胞的作用 ,用乙型肝炎病毒 (HBV)体外感染正常人骨髓中的淋巴细胞、中性粒细胞和单核细胞。培养 6小时至 3周后行动态观察 ,乙型肝炎核心抗原 (HBc Ag)阳性率表明 ,三种细胞均受到不同程度的感染 ,以中性粒细胞和单核细胞最敏感 ,其次为淋巴细胞 ,三者间有统计学差异 (P<0 .0 5或0 .0 1)。病毒感染程度随细胞增殖和时间延长而增强 ,无吞噬能力的淋巴细胞亦是如此。透射电镜观察感染后的中性粒细胞 ,6小时即出现线粒体和内质网的空泡变和髓样变性 ,2 4小时细胞膜破裂 ,72小时细胞表面重度破坏。表明病毒感染细胞有两种途径 ,一为病毒主动侵犯细胞 ,二为细胞吞噬病毒 ,在不同时期培养的上清液中均长时期检测到乙肝病毒表面抗原 (HBs Ag)、乙型肝炎病毒 e抗原 (HBe Ag)和乙型肝炎病毒 DNA (HBV- DNA)。

To make sure the effection of the hepatitis B virus (HBV) on the three kinds of cells in bone marrow Lymphocyte,neutrocyte and monocyte in bone marrow of normal human were infected by HBV in vitro.Through six different periods from six hours to three weeks,the positive rate of HbcAg of intercelluar indicated that the three kinds of cells were all infected in different extent.Neutrocyte and monocyte were more sensitive,followed by lymphocyte.There were significant difference among the three cells(P<0.05or<0.01).With proliferation of cells and prolongation of culture time,their infection degrees were enhanced.This included lymphocytes which were not phagocytically active infected neutrophils were observed by transmission electron microscope,mitochondria and endoplasmic reticula showed empty bleb denaturation and myelinated denaturation after 6 hours'culture.The cellular membranes were broken after 24 hours.The cellular surfaces were damaged seriously after 72 hours.These results indicates that there are two ways of HBV infecting cell,one is that virus invade cells activly,the other is cells engulf virus.HbsAg,HbeAgand HBV DNA could be detected in a lomg time in culturing supernatant fluid during different periods.