铝碳酸镁咀嚼片体外吸附胆酸的比较研究

Study on the bile acid-binding capacity between aluminum-magnesium hydroxycarbonate and sucralfate in vitro

目的 比较铝碳酸镁和硫糖铝在不同pH值条件下对胆酸的体外吸附程度。方法 利用胃十二指肠模型 ,在胃液中加入一定比例胆汁配成模拟胆汁返流的胃液。将模拟胃液调节至pH3,pH5 ,pH7,然后分别加入不同剂量铝碳酸镁和硫糖铝 ,37℃水浴孵育 1h。建立HPLC测定胆酸中各成分浓度的方法 ,并计算吸附率。结果 HPLC方法灵敏度高、特异性强。铝碳酸镁对总胆酸及各组分的吸附率均高于硫糖铝。结论 铝碳酸镁的层状网络结构对胆酸的吸附能力较强 。

OBJECTIVE To evaluate the bile acid binding capacities between aluminum magnesium hydroxycarbonate and sucralfate at different pH in vitro .METHODS An artificial gastrointestinal model was used. Different doses of aluminum magnesium hydroxycarbonate and sucralfate were added to the mixtures of gastric juice and bile juice with pH values of 3,5 and 7, respectively, and then incubated at 37℃ bath for 1 h.A HPLC assay was developed for the determination of five components in bile acid and their remain contents after incubation and adsorption.RESULTS The assay developed here was accurate, specific and simple.The adsorption rates of each component were higher for aluminum magnesium hydroxycarbonate than those of sucralfate at all of the pH values studied, so did the total adsorption rates.CONCLUSIONS:It demonstrated that the net like structure gel of aluminum magnesium hydroxycarbonate had considerable advantages in respect of bile acid consuming capacity. It may play a major role in treatment of patients with gastro esophageal reflux disease.