高效液相色谱法同时测定复方氯唑沙宗片中两组分血药浓度

Determination of two component concentrations of complex chlorzoxazone tablets in human serum by HPLC

目的 建立同时测定血清中复方氯唑沙宗片两组分浓度的HPLC。方法 血清样品经饱和硫酸锌溶液沉淀 ,乙醚提取 ,氮气挥干 ,加甲醇溶解进样 ,以HypersilODSC1 8色谱柱 ,甲醇 水 (4 5∶5 5 )为流动相 ,非那西丁为内标 ,在波长 2 80nm处检测。结果 氯唑沙宗在 1～ 32 μg·ml- 1 、对乙酰氨基酚在 1.2～ 38.4μg·ml- 1 浓度范围内 ,样品峰面积比与浓度呈良好线性关系 ,r氯 =0 .9994,r对 =0 .9999。最低检测浓度分别为 0 .5和 0 .6 μg·ml- 1 ,平均回收率分别为 95 .3 %和 91.6 %。RSD分别为 6 .0 % ,5 .1%。结论 此法可同时测定复方氯唑沙宗片两组分的浓度 。

OBJECTIVE To establish a HPLC method for the simultaneous determination of two component concentrations of complex chlorzoxazone tablets in human serum.METHODS The serum samples were precipitated with saturated Zinec sulfate solution,extracted with Ether,dried with Nitrogen gas,dissolved in Methanol and determined with HPLC.The Hypersil ODS C 18 column was used.The mobile phase consisted of methanol water (45∶55) phenacetinum was used as the internal standard.It was detected at 280 nm.RESULTS A linearity was obtained from 1.0 to 32.0 μg·ml -1 of chlorzoxazone ( r =0.9994) and from 1.2 to 38.4 μg·ml -1 of acetaminophen ( r =0.9999),respectively.The limits of detection were 0.5 and 0.6 μg·ml -1 ,respectively.Their average recoveries were 95.3% and 91.6%,respectively.The precision ( RSD ) were 6.0% and 5.1%,respectively.CONCLUSION This study provided a simple and reliable assay which can be used to simultaneously determine two component concentrations of complex chlorzoxazone tablets in human serum and to reseach their pharmacokinetics and bioavailability.