肺癌组织中S-100阳性树突状细胞图像定量研究

IMAGE QUANTITATIVE STUDY OF S-100 POSITIVE DENDRITIC CELLS IN LUNG CARCINOMA

目的 :为研究肺癌组织中树突状细胞的数量及分布特点。方法 :用 S- 10 0蛋白免疫组织化学染色对 37例肺癌及癌周组织和 12例正常肺组织中的树突状细胞进行原位观察及图像定量分析。结果 :肺癌组织和癌周组织中树突状细胞数密度和面密度明显大于对照组 (P <0 .0 1) ,且单个细胞的长径和面积大于对照组 (P <0 .0 5 ) ,肺癌中无淋巴结转移组的树突状细胞多于有转移组 (P <0 .0 5 ) ,癌周淋巴细胞浸润的程度与树突状细胞的数量密切相关 ,但树突状细胞的数量与肺癌的组织类型无关。结论 :肺癌的树突状细胞数量明显增多且体积增大 ,其数量与淋巴结转移和癌周淋巴细胞多少密切相关。

Objective:To study the quantity and distribution of dendritic cells (DC) in lung carcinoma Methods:DC in paraffin embedded tissue was stained with immunohistochemical method using S 100 protein antibody from 37 cases of lung carcinoma The number and area density of DC were measured by image analysis system Twelve normal lung tissues served as control Results:Compared with control group,the number and area density of DC in lung carcinoma was greater The cells morphological size was larger and longer in cancer than normal The number of DC in lymph node metastases group was higher than no metastases group The intensity of lymphocyte infiltration around the tumor was closely related to the degree of DC infiltration Conclusion:The number and cell's size of DC was increased in lung carcinoma and related to metastases in lymph node and the intensity of lymphocyte infiltration