免疫乳清对葡萄糖基转移酶活性的影响

The effect of immune bovine whey on cell associated glucosyltransferase activity of Streptococcus mutans

目的 了解免疫乳清对变形链球菌葡萄糖基转移酶 (glucosyltransferase ,GTF)活性的影响。方法 免疫乳清来源于GTF过表达株B 2 9 33免疫孕牛中获得的牛奶。对照乳清从未免疫孕牛中获得。用gtfB基因缺陷株B 2 9吸收免疫乳清中的抗体获得免疫吸收乳清。采用蒽酮定糖法检测3种乳清在 5 0、70及 90 μl3种剂量条件下对GTF合成水不溶性葡聚糖的影响。 结果 对照乳清具有增强酶活性的作用 ,可使酶活性增强 3倍以上。免疫吸收乳清可部分抑制这种增强作用。免疫乳清可完全抑制乳清对酶活性的增强作用 ,并在此基础上抑制GTF的活性。结论 免疫乳清可以有效抑制GTF合成水不溶性葡聚糖。

Objective To study the effect of immune bovine whey on cell associated glucosyltransferase (GTF) activity of S.mutans MT8148. Methods The immune milk was collected from cows immunized with cell associated GTF overexpression strain B 29 33 of S.mutans MT8148. The control milk was from non immunized cows. The immune absorbed whey was gotten from immune bovine whey which was absorbed with lyophilized Formalin killed B 29 whole cells. Three kinds of whey were subdivided into three groups: 50 μl、70 μl、90 μl. The content of insoluble glucan was estimated colorimetrically by anthrone method. Results The control bovine whey had an enhancing GTF activity (407 00%～485.62%). The immune absorbed whey inhibited partly the enhancing GTF activity (208.74%～273 00%). The immune whey inhibited significantly the GTF activity (70 24%～38.62%) and the inhibition showed a tendency to depend on doses. Conclusions The immune bovine whey inhibits significantly the cell associated GTF activity of S.mutans MT8148.