异丙肾上腺素对大鼠心肌细胞凋亡相关基因表达的影响

EFFECTS OF ISOPRENALINE ON APOPTOSIS RELATED GENE EXPRESSION IN RAT MYOCARDIUM CELLS

目的 研究异丙肾上腺素 (isoprenaline,Iso)诱导大鼠急性心肌缺血与心肌细胞凋亡相关基因表达的关系。方法 sc大剂量Iso建立大鼠急性心肌缺血模型。RT PCR法检测凋亡相关基因Fas,CPP32 ,Bcl 2和Bax在心肌的表达。并用同样方法 ,观察苦豆碱 (aloperine,Alo ,2 0mg·kg-1)和普萘洛尔 (propranolol,Prop ,2mg·kg-1)对急性心肌缺血大鼠心肌细胞凋亡相关基因表达的影响。结果 急性心肌缺血模型大鼠心肌的Fas,CPP32和Bcl 2基因表达显著增加 ,Bax变化不明显 ;Alo和Prop能降低缺血心肌中Fas和CPP32基因的表达 ,增加Bcl 2基因的表达 ,且Prop对Bax表达有下调作用。结论 sc大剂量Iso可诱导大鼠心肌细胞凋亡相关基因异常表达 ;

AIM To study the relationship between acute myocardial ischemia rat induced by isoprenaline (Iso) and expression of some apoptosis related genes in myocardium. METHODS Acute myocardial ischemic rat model was induced by subcutaneous injection of large doses of Iso. The myocardial expression of apoptosis related genes, Fas, CPP32, Bcl 2 and Bax, were detected with RT PCR method. The influences of aloperine (Alo, 20 mg·kg -1 ) and propranolol (Prop, 2 mg·kg -1 ) on apoptosis related genes were also investigated by the same method. RESULTS Myocardial expression of Fas, CPP32 and Bcl 2 were increased in acute myocardial ischemic rats, but no significance was detected in Bax expression. Both Alo and Prop were shown to decrease the expression of Fas and CPP32, and increase expression of Bcl 2. Prop also showed down regulated effect on expression of Bax. CONCLUSION Subcutaneous injection of large doses of Iso was shown to induce expressions of apoptosis related genes in rat myocardium, which were also influences by Alo and Prop.