亚临界水色谱的初步研究

FOUNDATIONAL STUDY OF SUBCRITICAL WATER CHROMATOGRAPHY

目的 探讨亚临界水色谱法用填充柱和毛细管柱分离中、高极性化合物的色谱规律。方法 用氢焰离子化检测器 (FID)分别在填充柱和毛细管柱上建立了亚临界水色谱系统。在C18柱上分离了 6种不同的醇 ,考察了温度变化对不同极性醇类色谱行为的影响 ;在毛细管柱上 ,考察了温度和压力变化对苯甲酸和人参Rg1色谱行为的影响。结果 温度升高 ,流动相的极性降低 ,被分离组分的保留时间缩短 ,峰形变窄 ,拖尾改善。结论 程序升温可以选择性地分离不同极性的化合物 ;毛细管柱更具耐热性 。

AIM To study the subcritical water chromatographic discipline of compounds with medium and high polarity on the capillary and packed column. METHODS A packed and capillary subcritical water chromatography FID detection system was built up. Six alcohols were well separated on a C 18 column and the influence of temperature on chromatographic behavior of alcohols with different polarity was studied. The influence of temperature and pressure on chromatographic behavior of benzoic acid and gensenoside Rg1 on a SE 54 capillary column were also studied. RESULTS Increasing temperature resulted in the decreasing polarity of mobile phase, shortened retention times of components separated, narrowed peak shape and improved tailing as well. CONCLUSION Temperature programming could be used successfully to separate compounds with various polarity selectively. Thermostable capillary column could enlarge the range of temperature even more.